Reduction of allergenicity of litchi chinensis flowers pollen protein
Background: Allergy to pollen from gymnosperms is well documented in the west. The objective was to define the allergologic protein from Litchi chinensis (Litchi) pollen and conjugate the protein with polysaccharides by Maillard reaction to reduce the allergic effect of that protein. Methods: Total...
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my.umk.eprints.76752022-05-23T10:21:40Z http://discol.umk.edu.my/id/eprint/7675/ Reduction of allergenicity of litchi chinensis flowers pollen protein Ranajit Kumar Shaha Nitai Roy G.Talukdar Background: Allergy to pollen from gymnosperms is well documented in the west. The objective was to define the allergologic protein from Litchi chinensis (Litchi) pollen and conjugate the protein with polysaccharides by Maillard reaction to reduce the allergic effect of that protein. Methods: Total soluble proteins were extracted from the pollen of Litchi flower pollen and subjected to ammonium sulphate precipitation at 80% saturation. Pollen antigen from Litchi chinensis (Litchi) was prepared by gel cutting method and characterized by biochemical and designated by LFPP. The homogeneity of this protein was demonstrated by a single band on SDSPAGE. The protein then conjugated with galactomannan through Maillard Reaction. The resulting purified pollen protein and conjugated protein were administrered to the swiss albino mice as amount of 5.8mg/kg body weight. Results: The total protein was then separated on a 12% SDS-Polyacrylamide gel which revealed 5 bands between molecular weight range of 29 kDa and 69 kDa. Each band was recovered from the gel by electroelution and sent for skin tests. 28 kDa proteins was the only allergenic protein while others were not shown reactivity in patients. Intraperitoneal injection of the purified protein (LFPP) caused a significant rise in the levels of neutrophils (38-81%) and eosinophils (3-14%) compared to control (P<0.001) whereas conjugated protein caused only a 2% increase of both neutrophils and eosinophils level. On the other hand treatment with LFPPgalactomannan conjugate causes no such change in physical appearance with eosinophils and neutrophils level. Conclusion: The present study demonstrates that the protein extracted and purified from Litchi flowers pollen has been recognized as a new allergen from Bangladesh for the first time and the allergic effects can be reduced by conjugation with polysaccharides. 2012-04 Non-Indexed Article NonPeerReviewed Ranajit Kumar Shaha and Nitai Roy and G.Talukdar (2012) Reduction of allergenicity of litchi chinensis flowers pollen protein. Journal of Advanced Laboratory Research in Biology, 3 (2). pp. 110-119. ISSN 0976-7614 http://www.jalrb.org/images/VOL-III-ISUEE-II-APR2012/109-119REDUCTION%20OF%20ALLERGENICITY%20OF%20LITCHI%20CHINENSIS%20FLOWERS%20POLLEN%20PROTEIN.pdf |
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Background: Allergy to pollen from
gymnosperms is well documented in the
west. The objective was to define the
allergologic protein from Litchi chinensis
(Litchi) pollen and conjugate the protein
with polysaccharides by Maillard reaction
to reduce the allergic effect of that
protein.
Methods: Total soluble proteins were
extracted from the pollen of Litchi flower
pollen and subjected to ammonium
sulphate precipitation at 80% saturation.
Pollen antigen from Litchi chinensis
(Litchi) was prepared by gel cutting
method and characterized by biochemical
and designated by LFPP. The
homogeneity of this protein was
demonstrated by a single band on SDSPAGE.
The protein then conjugated with
galactomannan through Maillard
Reaction. The resulting purified pollen
protein and conjugated protein were
administrered to the swiss albino mice as
amount of 5.8mg/kg body weight.
Results: The total protein was then
separated on a 12% SDS-Polyacrylamide
gel which revealed 5 bands between
molecular weight range of 29 kDa and 69
kDa. Each band was recovered from the
gel by electroelution and sent for skin
tests. 28 kDa proteins was the only
allergenic protein while others were not
shown reactivity in patients.
Intraperitoneal injection of the purified
protein (LFPP) caused a significant rise
in the levels of neutrophils (38-81%) and
eosinophils (3-14%) compared to control
(P<0.001) whereas conjugated protein
caused only a 2% increase of both
neutrophils and eosinophils level. On the
other hand treatment with LFPPgalactomannan
conjugate causes no such
change in physical appearance with
eosinophils and neutrophils level.
Conclusion: The present study
demonstrates that the protein extracted
and purified from Litchi flowers pollen
has been recognized as a new allergen
from Bangladesh for the first time and the
allergic effects can be reduced by
conjugation with polysaccharides. |
format |
Non-Indexed Article |
author |
Ranajit Kumar Shaha Nitai Roy G.Talukdar |
spellingShingle |
Ranajit Kumar Shaha Nitai Roy G.Talukdar Reduction of allergenicity of litchi chinensis flowers pollen protein |
author_facet |
Ranajit Kumar Shaha Nitai Roy G.Talukdar |
author_sort |
Ranajit Kumar Shaha |
title |
Reduction of allergenicity of litchi chinensis flowers pollen protein |
title_short |
Reduction of allergenicity of litchi chinensis flowers pollen protein |
title_full |
Reduction of allergenicity of litchi chinensis flowers pollen protein |
title_fullStr |
Reduction of allergenicity of litchi chinensis flowers pollen protein |
title_full_unstemmed |
Reduction of allergenicity of litchi chinensis flowers pollen protein |
title_sort |
reduction of allergenicity of litchi chinensis flowers pollen protein |
publishDate |
2012 |
url |
http://discol.umk.edu.my/id/eprint/7675/ http://www.jalrb.org/images/VOL-III-ISUEE-II-APR2012/109-119REDUCTION%20OF%20ALLERGENICITY%20OF%20LITCHI%20CHINENSIS%20FLOWERS%20POLLEN%20PROTEIN.pdf |
_version_ |
1763303875063840768 |
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13.160551 |