Water microbe screening from river bank along Lata Janggut, Kelantan.
Water microbiology is about the microorganisms that live in the water. The significance of this research is to determine the diversity of microbes in water. This study aim to screen and identify water microbes from Lata Janggut, Kelantan. The research started from taking the water samples from Lata...
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| Main Author: | |
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| Format: | Undergraduate Final Project Report |
| Language: | English |
| Published: |
2017
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| Online Access: | http://discol.umk.edu.my/id/eprint/7208/1/NOR%20SHAKIRAH%20RAMLI.pdf http://discol.umk.edu.my/id/eprint/7208/ |
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| Summary: | Water microbiology is about the microorganisms that live in the water. The significance of this research is to determine the diversity of microbes in water. This study aim to screen and identify water microbes from Lata Janggut, Kelantan. The research started from taking the water samples from Lata Janggut, isolation of bacteria, bacteria morphology identification, DNA extraction, gel electrophoresis and polymerase chain reaction (PCR). The isolation of water microbes is using enrichment culture that contains Nutrient Agar (NA) media, Luria Bertani (LB) broth, and water sample from serial dilution. The result from Gram staining showed, 6 out of 8 isolates were Gram positive while the other 2 were Gram negative after staining. The isolated colonies morphology are both coccii and bacilli. The samples were measured with UV spectrophotometer and Nanodrop Spectrophotometer to obtain their optical density. The research was continued with 1% agarose gel electrophoresis (AGE) which the DNA bands can be visualized under UV light. In Polymerase Chain Reaction (PCR), multiple bands were formed. Hence, future application for screening and identification of water microbes will be investigated further. |
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