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Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants

Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneid...

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Main Author: Umi Nor Zarina Othman
Format: Undergraduate Final Project Report
Published: 2016
Online Access:http://discol.umk.edu.my/id/eprint/6622/
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spelling my.umk.eprints.66222022-05-23T08:48:21Z http://discol.umk.edu.my/id/eprint/6622/ Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants Umi Nor Zarina Othman Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneidensis MR-1 respiratory mutants unable to reduce Nitrate (N0₃­) as anaerobic electron acceptor. S.oneidensis nitrate-reductant deficient mutants screening technique was based on the observation that wild-type S. oneidensis produced a red precipitate on its colony surface during screening test, while colonies arising from mutagenized cells did not changed the color at all. The cell density of mutagenized cells 0.2396 (±.152) is lower compared to wild type control 0.3318 (±.576). This report describes the design of rapid screening technique and its application to identify mutants in a metal-reducing member of the genus Shewanella. 2016 Undergraduate Final Project Report NonPeerReviewed Umi Nor Zarina Othman (2016) Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneidensis MR-1 respiratory mutants unable to reduce Nitrate (N0₃­) as anaerobic electron acceptor. S.oneidensis nitrate-reductant deficient mutants screening technique was based on the observation that wild-type S. oneidensis produced a red precipitate on its colony surface during screening test, while colonies arising from mutagenized cells did not changed the color at all. The cell density of mutagenized cells 0.2396 (±.152) is lower compared to wild type control 0.3318 (±.576). This report describes the design of rapid screening technique and its application to identify mutants in a metal-reducing member of the genus Shewanella.
format Undergraduate Final Project Report
author Umi Nor Zarina Othman
spellingShingle Umi Nor Zarina Othman
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
author_facet Umi Nor Zarina Othman
author_sort Umi Nor Zarina Othman
title Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
title_short Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
title_full Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
title_fullStr Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
title_full_unstemmed Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
title_sort design of a rapid screening technique for identification of shewanella oneidensis nitrate-reductant deficient mutants
publishDate 2016
url http://discol.umk.edu.my/id/eprint/6622/
_version_ 1763303723511054336
score 13.18916

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