Isolation and characterization of iron-reducing bacteria from freshwater sediments at agropark,UMK, Kelantan and Gunung Yong Beiar, Perak

• Main Author: Andi Mariati Andi Radi
• Format: Undergraduate Final Project Report
• Published: 2016
• Online Access: http://discol.umk.edu.my/id/eprint/6528/

lron reducing bacteria is one of the microorganisms that exist in freshwater sediments. Freshwater sediments contains various microorganisms which are beneficial and some could be pathogenic. Several significances of this research such as in bioremediation, for example reduction of Uranium(Vl) to Uranium(lV), application in azo-dyes industry and in iron biogeochemical cycles. This thesis aim to isolate and characterize iron reducing bacteria from selected fresh water sediments at Agropark,UMK, Kelantan and Ladang Sayur and Kem Tudung Periuk which are located at Gunung Yong Belar, Perak. The isolation is by using enrichment culture which contains Luria Bertani (LB) media, Fe(ll) citrate and samples. 9 selected colonies were chose, 7 out of 9 isolates were Gram positive while the other 2 were Gram negative. The isolate‘s morphologies are both rod and cocii. Besides that, 8 out of 9 isolates are catalase positive and the other were catalase negative. 7 out of 9 isolates are Oxidase positive and the other 2 isolates were Oxidase negative. Fe(ll) concentration were measured by using Ferrozine method where sample MAR-01 and MAR-02 from Gunung Yong Belar shows the highest content of Fe(ll) concentration. This study reports that there are abundance microorganisms presents in freshwater sediments including iron reducing bacteria which is beneficial towards the environment.