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Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers

Large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. This study was conducted to generate RAPD markers from six selected banana cultivars collected from five different area of Peninsular Malaysia. The annealing temperature used for the R...

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Main Author: Norhafizah Ghazali
Format: Undergraduate Final Project Report
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/5997/
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spelling my.umk.eprints.59972022-05-23T08:44:24Z http://discol.umk.edu.my/id/eprint/5997/ Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers Norhafizah Ghazali Large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. This study was conducted to generate RAPD markers from six selected banana cultivars collected from five different area of Peninsular Malaysia. The annealing temperature used for the RAPD analysis was 36°C. The RAPD fingerprinting of these banana varieties was carried out by three primers; OPA-13, OPB-10, and OPI-16. The RAPD profiles showed wide array of weak and strong DNA bands that could due to the low amount of DNA content in the sample used. RAPD primers produced 23 amplified fragments varying from 220 by to 1500 by in size. 9% of the amplification bands were monomorphic while 91 % were polymorphic bands. Only primers OPB-10 and OPI-16 were able to produce both monomorphic and polymorphic bands. 2014 Undergraduate Final Project Report NonPeerReviewed Norhafizah Ghazali (2014) Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers. Undergraduate Final Project Report thesis, Faculty Of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. This study was conducted to generate RAPD markers from six selected banana cultivars collected from five different area of Peninsular Malaysia. The annealing temperature used for the RAPD analysis was 36°C. The RAPD fingerprinting of these banana varieties was carried out by three primers; OPA-13, OPB-10, and OPI-16. The RAPD profiles showed wide array of weak and strong DNA bands that could due to the low amount of DNA content in the sample used. RAPD primers produced 23 amplified fragments varying from 220 by to 1500 by in size. 9% of the amplification bands were monomorphic while 91 % were polymorphic bands. Only primers OPB-10 and OPI-16 were able to produce both monomorphic and polymorphic bands.
format Undergraduate Final Project Report
author Norhafizah Ghazali
spellingShingle Norhafizah Ghazali
Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
author_facet Norhafizah Ghazali
author_sort Norhafizah Ghazali
title Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
title_short Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
title_full Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
title_fullStr Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
title_full_unstemmed Genetic analysis of selected banana cultivars in Peninsular Malaysia by using rapd markers
title_sort genetic analysis of selected banana cultivars in peninsular malaysia by using rapd markers
publishDate 2014
url http://discol.umk.edu.my/id/eprint/5997/
_version_ 1763303633496047616
score 13.18916

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