Comparative studies of rubber latec c-serum secreted from young, mature and old rubber trees (hevea brasiliensis)

• Main Author: Chan, Chin Hui
• Format: Undergraduate Final Project Report
• Published: 2013
• Online Access: http://discol.umk.edu.my/id/eprint/5755/

Rubber latex C-Serum from young, mature and old rubber tree (Hevea brasiliensis) (in terms of productivity) were isolated. The protein concentration of C-Serum were determined using Bradford assay. SDS-PAGE (Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis) is used to compare the protein profile of these three subjects. Gravity gel filtration used to separate the protein in C-Serum based on their molecular size and SDS-PAGE analysis is done on the collected fraction with high peak. Bradford protein concentration assay showed that rubber latex C-Serum from mature rubber tree contained highest protein concentration which is 12.683 mg/ml, following by young rubber tree and old rubber tree which is 7.368 mg/ml and 3.267 mg/ml respectively. The SDS-PAGE analysis result showed that there no much difference between rubber latex C-Serum from young, mature and old rubber trees. The SDS-PAGE analysis showed there are some similarity in the protein band identified such as prenyltransferase with molecular weight of 38 kDa, PPO-I and PPO-II with molecular weight of 32-34 kDa, and lysozome with molecular weight of 26-27.5 kDa.