Total plate count of escherichia coli isolated from smoked asian clam, corbicula fluminea with emphasis on its antibiogram

• Main Author: Siti Hajar Ab Rahim
• Format: Undergraduate Final Project Report
• Published: 2013
• Online Access: http://discol.umk.edu.my/id/eprint/5749/

In this study, total plate counts of Escherichia coli isolated from Smoked Asian Clam.Corbicula ,fluminea with emphasis on its antibiogram are described. Smoked Asian Clams were known as a popular side dishes in Malaysia especially in state of Kelantan. Eventhough there was increasing rate of gastrointestinal disease and E. coli infections suffered among the peoples who consumed the Smoked Asian Clams. there was no detail database information on antibiogram profile of E. coli from Smoked Asian Clams had been recorded. Therefore, this study was carried out to investigate the total bacterial colony of E. coli in Smoked Asian Clams and to determine the effectiveness of 19 antibiotics against isolated E. coli. The susceptibility testing was pc:thorned using standard disk diffusion method against 19 tested antibiotics namely sulphamethoxazole (25 μg), oleondomycin (15 μg), florfenicol (30 μg), tetracycline (30 μg), spiramycin (100 μg), colistine sulphate (25 μg), flumequine (30 μg), ampicillin (10 μg), oxolinic acid (2 μg), novobiocin (30 μg), fosfomycin (50 μg). erythromycin (15 μg), nalidixic acid (30 μg), doxycycline (30 μg), chloramphenicol (30 μg), compound sulphonamides (300 μg), lincomycin (15 μg), amoxycillin (25 μg). gentamycin (120 μg). The isolates were highly resistance to nine antibiotics (novobiocin, ampicillin, lincomycin, amoxycillin, colistin sulphate, spiramycin. oleondomycin, erythromycin, sulphamethoxazole) and as well as highly sensitive to another four antibiotics (chloromphenicol, oxolinic acid, nalidixic acid, flumequine). Most of the isolates showed multiple resistance toward 13 out of 19 tested antibiotics. The bacteria isolate performed haemolytic property after 72h of incubation on 5% sheep blood agar. Multiple antibiotic resistance (MAR) indicate the high exposure of Smoked Asian Clams (C. fluminea) toward tested antibiotics. It also indicated the level of contamination of water source with antibiotics residues.