Effects of Plant Growth Regulators on Callus Induction of Clitorea ternatea

• Main Author: Nor Ain Md Sabri
• Format: Undergraduate Final Project Report
• Language: English
• Published: 2019
• Online Access: http://discol.umk.edu.my/id/eprint/4637/1/NOR%20AIN%20BINTI%20MD%20SABRI.pdf; http://discol.umk.edu.my/id/eprint/4637/

Clitoria ternatea is an herb plant that have valuable medicinal value. The properties of the plant that able to cure diseases shows the potential of this plant being over exploit due to the changing trend in life style and health. C. ternatea commonly used in traditional medicine and had also been used in modern treatment. In this project, the used of the two different Plant Growth Regulator (PGR) 6-benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) at several different combination concentration together with the application of thin cell layer (TCL) method transverse TCL (tTCL), longitudinal TCL (lTCL) and Square 1cm2 are applied as the variable to bring out the callus. The best callus induction treatment in this experiment is 2 mg/L BAP + 0.1 mg/L IAA with value 56.95 mg and the best TCL method used is tTCL with value 18.52 mg. Thus, this plant have a potential to produce secondary metabolite through tissue culture method.