Isolation of potential phages against Aeromonas veronii

• Main Author: Mclaren Robert
• Format: Undergraduate Final Project Report
• Language: English
• Published: 2023
• Online Access: http://discol.umk.edu.my/id/eprint/14150/1/D19A0012%20MCLAREN%20ROBERT.docx.pdf; http://discol.umk.edu.my/id/eprint/14150/

An abstract of the research paper was presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement for the course DVT 55204 – Research Project. In today’s era of emerging antimicrobial resistance, there has been an increased emphasis on bacteriophages, though it is surprising that bacteriophages against Aeromonas veronii is not much studied. Therefore, this study focuses on the isolation of potential bacteriophages against Aeromonas veronii from sewage water, river and soil in Kota Bharu, Kelantan.. The research involved the collection of environmental samples such as water and soil, followed by the isolation of potential phages using A. veronii strains as hosts. Enrichment technique using pond water along with bacterial culture was employed for the isolation of phage followed by double layered agar plaque assay for titre enumeration. In this study, two bacteriophages were isolated from Universiti Malaysia Kelantan (UMK) and Sungai Pengkalan Chepa (SPC) unfortunately only one bacteriophage sample were further studies which is sample from Universiti Malaysia Kelantan (UMK) due to time constraint. The isolated phages underwent host range determination and plaque forming units were conducted to enumerate the titer of the isolated bacteriophage and the highest countable dilution is 10-5 and the titer is 1.25 x 10-3 PFU/mL. These findings contribute valuable insights into the potential use of bacteriophages as targeted agents for controlling Aeromonas veronii infections. Further exploration of these isolated phages could pave the way for the development of tailored phage therapies, offering a precise and effective approach to combating A. veronii-related illnesses. Keywords: Aeromonas veronii, Bacteriophage, antimicrobial resistance