Isolation, identification, and amr pattern of e. Coli isolates From pellet-fed and chicken-offal fed african catfish (clarias gariepinus)

• Main Author: Nur Syaza Syazwani Mohd Azhar
• Format: Undergraduate Final Project Report
• Language: English
• Published: 2022
• Online Access: http://discol.umk.edu.my/id/eprint/10650/1/NUR%20SYAZA%20SYAZWANI%20%20MOHD%20AZHAR.pdf; http://discol.umk.edu.my/id/eprint/10650/

Escherichia coli (E. coli) belongs to the family Enterobacteriaceae and is a commensal bacteria of the gastrointestinal tract of African catfish (Clarias gariepinus). This is a preliminary study to determine the antimicrobial resistance (AMR) pattern of E. coli in African catfish as well as the possible relationship between antibiotic resistance and type of aquaculture feed. Five pellet-fed and five chicken offal-fed African catfishes were obtained for the collection of intestinal mucosa samples for isolation and identification of E. coli, and an AMR pattern of isolated E. coli was executed. Results revealed three E. coli isolates from the pellet-fed catfish group were resistant to tetracycline; one of the isolates was simultaneously resistant to chloramphenicol. All E. coli isolates from the chicken offal-fed catfish group were resistant to at least one selected antibiotic, and all isolates were 100% resistant against tetracycline and amoxicillin. There is an occurrence of multidrug resistance for all E. coli isolates from the chicken offal-fed catfish group. The study supports the notion that E. coli isolated from chicken offal-fed catfish are more resistant to selected antimicrobials than commercial pellet-fed catfish which could suggest influence of type of feed received. Keywords: African catfish, Antimicrobial resistance, E. coli, Feed