Cloning and expression of thermostable protease gene from Bacillus Subtilis 50a in Escherichpa Coli BL21 (DE3) pLysS
A thermostable protease producer was isolated from a hot spring in Lojing Highlands, Kelantan. It was identified as Bacillus subtilis 50a based on morphology and 16S rRNA sequencing analysis. Its native protease displayed the maximal activity at pH 9 and showed high optimum temperature of 70˚C(18 U/...
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Format: | UMK Etheses |
Published: |
2012
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Online Access: | http://discol.umk.edu.my/id/eprint/10347/ |
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