Effects of monoterpenes on glucose uptake and lipid metabolism in 3T3-L1 adipocytes / Tan Xien Cherh
Various strategies have been adopted to combat complications caused by Type 2 diabetes mellitus (T2DM) and diet control is one of them. Monoterpenes, the major constituents of essential oils, are synthesized and widely used as artificial food flavours. The aims of the present study were to assess...
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| Format: | Thesis |
| Published: |
2016
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| Online Access: | http://studentsrepo.um.edu.my/7060/4/tan_xien.pdf http://studentsrepo.um.edu.my/7060/ |
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| Summary: | Various strategies have been adopted to combat complications caused by Type 2
diabetes mellitus (T2DM) and diet control is one of them. Monoterpenes, the major
constituents of essential oils, are synthesized and widely used as artificial food flavours.
The aims of the present study were to assess the effects of twelve monoterpenes on
antioxidant capacity, glucose uptake and lipid metabolism. Antioxidant capacity of
monoterpenes were assessed using to 2, 2-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2�-
azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. In
vitro alpha-amylase and alpha-glucosidase inhibitory assays were used to assess the
potential anti-hyperglycaemic property of monoterpenes. The 3T3-L1 cellular model
was used to assess the effects of monoterpenes on adipocyte differentiation, lipid
accumulation, glucose uptake and lipolysis. Gene expression study was conducted to
determine the potential mechanisms involved in the above mentioned processes. Results
showed that the monoterpenes used in the current study exerted low DPPH and ABTS
radical scavenging activity even at high concentrations. Some monoterpenes inhibited
alpha-glucosidase activities and stimulated glucose uptake and lipolysis in 3T3-L1
adipocytes. (R)-(+)-Limonene stimulated both glucose uptake (17.4%) and lipolysis
(17.7%); in parallel, the mRNA expressions of glucose transporter 1 (GLUT1) was
upregulated, glucose transporter 4 (GLUT4) was unaffected, and mRNA expression of
adipose triglyceride lipase (ATGL) was suppressed. Oil Red O quantitative results
showed that low level oxidative stress induced by glucose oxidase was able to stimulate
both adipogenesis and lipid accumulation in 3T3-L1 adipocytes though mRNA
expression of glutathione peroxidase (GPx) remained unaffected. The effect of glucose
oxidase in adipogenesis and lipogenesis was not affected by the addition of
monoterpenes. Taken together, the selected monoterpenes may not confer strong
iv
protection against free radicals but nevertheless, their positive influence on lipid and
glucose metabolism may have potential in the control of obesity and T2DM |
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