Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran
Blastocystis sp. is an enteric protozoan organism, found in the intestinal tract of a wide range of animals and humans. The parasite is often incriminated to cause symptoms such as diarrhoea, abdominal pain, bloating and flatulence. Many publications have highlighted the high prevalence of Blasto...
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my.um.stud.69662020-01-18T02:53:32Z Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran Hemalatha, Chandrasekaran R Medicine (General) Blastocystis sp. is an enteric protozoan organism, found in the intestinal tract of a wide range of animals and humans. The parasite is often incriminated to cause symptoms such as diarrhoea, abdominal pain, bloating and flatulence. Many publications have highlighted the high prevalence of Blastocystis sp. in various animal hosts, however, only very few studies have been conducted in Malaysia. Therefore, this study aimed to screen a range of animals in Malaysia for Blastocystis sp. infection which could be a reservoir for human infection when in close association. High prevalence of Blastocystis sp. were seen in the ostriches and pigs with 100% positive respectively and for the first time, subtype 6 Blastocystis sp. was seen in ostrich isolates. Previous findings have described on either cysts or vacuolar forms, however, these are confined to limited animals. This study has provided evidence that the parasite’s thick cyst wall is primarily responsible for causing the infection in rats and the thickness of the cyst wall corresponded to the number of days taken to cause the infection. This is the first study to establish a schematic drawing which provides a key-like guidance to differentiate the morphology of the vacuolar and cystic forms of Blastocystis sp. isolated from animals which can provide information for source tracking. The worldwide distribution and increase in the infection rate demonstrate the zoonotic potential and the parasite’s low host-specificity. The present study also suggests that Blastocystis sp. exhibits low host specificity and the possibility of human to animal cross-infectivity cannot be ruled out. Despite studies reporting on the ability of Blastocystis sp. to be invasive, there have been no studies to assess the histopathological changes in rats infected with Blastocystis ST3 isolated from symptomatic and asymptomatic patients. The gross changes in the histopathology and elevated level of Abstract iv serum amylase in symptomatic rats despite being infected with the same subtype from symptomatic and asymptomatic human isolates further confirm the pathogenic role of Blastocystis sp. Very few studies have described the transformational details during the excystation of Blastocystis sp. cysts. To date, there has been no comparison on the excystation rates and transformational changes between parasites from symptomatic and asymptomatic isolates. This present study demonstrated marked differences in the excystation process between Blastocystis sp. from asymptomatic and symptomatic isolates. The present study has shown that extensive mucosal sloughing occurred in the intestines of rats infected with asymptomatic isolate which revealed that Blastocystis sp. excysts and proliferate soon after infection compared to symptomatic group. This finding concurred with previous studies that showed profileration to be the highest in invitro cultures of the asymptomatic isolates between days 3 and 6. Results also showed that rapid excystation and proliferation in asymptomatic isolates may cause heightened symptoms immediately to the host once infected. 2016 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/6966/1/hema.pdf Hemalatha, Chandrasekaran (2016) Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/6966/ |
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R Medicine (General) Hemalatha, Chandrasekaran Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
description |
Blastocystis sp. is an enteric protozoan organism, found in the intestinal tract of a
wide range of animals and humans. The parasite is often incriminated to cause
symptoms such as diarrhoea, abdominal pain, bloating and flatulence. Many
publications have highlighted the high prevalence of Blastocystis sp. in various animal
hosts, however, only very few studies have been conducted in Malaysia. Therefore, this
study aimed to screen a range of animals in Malaysia for Blastocystis sp. infection
which could be a reservoir for human infection when in close association. High
prevalence of Blastocystis sp. were seen in the ostriches and pigs with 100% positive
respectively and for the first time, subtype 6 Blastocystis sp. was seen in ostrich isolates.
Previous findings have described on either cysts or vacuolar forms, however, these are
confined to limited animals. This study has provided evidence that the parasite’s thick
cyst wall is primarily responsible for causing the infection in rats and the thickness of
the cyst wall corresponded to the number of days taken to cause the infection. This is
the first study to establish a schematic drawing which provides a key-like guidance to
differentiate the morphology of the vacuolar and cystic forms of Blastocystis sp.
isolated from animals which can provide information for source tracking. The
worldwide distribution and increase in the infection rate demonstrate the zoonotic
potential and the parasite’s low host-specificity. The present study also suggests that
Blastocystis sp. exhibits low host specificity and the possibility of human to animal
cross-infectivity cannot be ruled out. Despite studies reporting on the ability of
Blastocystis sp. to be invasive, there have been no studies to assess the histopathological
changes in rats infected with Blastocystis ST3 isolated from symptomatic and
asymptomatic patients. The gross changes in the histopathology and elevated level of
Abstract
iv
serum amylase in symptomatic rats despite being infected with the same subtype from
symptomatic and asymptomatic human isolates further confirm the pathogenic role of
Blastocystis sp. Very few studies have described the transformational details during the
excystation of Blastocystis sp. cysts. To date, there has been no comparison on the
excystation rates and transformational changes between parasites from symptomatic and
asymptomatic isolates. This present study demonstrated marked differences in the
excystation process between Blastocystis sp. from asymptomatic and symptomatic
isolates. The present study has shown that extensive mucosal sloughing occurred in the
intestines of rats infected with asymptomatic isolate which revealed that Blastocystis sp.
excysts and proliferate soon after infection compared to symptomatic group. This
finding concurred with previous studies that showed profileration to be the highest in invitro
cultures of the asymptomatic isolates between days 3 and 6. Results also showed
that rapid excystation and proliferation in asymptomatic isolates may cause heightened
symptoms immediately to the host once infected. |
format |
Thesis |
author |
Hemalatha, Chandrasekaran |
author_facet |
Hemalatha, Chandrasekaran |
author_sort |
Hemalatha, Chandrasekaran |
title |
Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
title_short |
Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
title_full |
Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
title_fullStr |
Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
title_full_unstemmed |
Studies on prevalence of Blastocystis sp. in animals and experimental infection in-vivo / Hemalatha Chandrasekaran |
title_sort |
studies on prevalence of blastocystis sp. in animals and experimental infection in-vivo / hemalatha chandrasekaran |
publishDate |
2016 |
url |
http://studentsrepo.um.edu.my/6966/1/hema.pdf http://studentsrepo.um.edu.my/6966/ |
_version_ |
1738505974627434496 |
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13.19449 |