Cloning, expression and functional study of specific DNA markers for Salmonella Typhi / Noradilin binti Abdullah

• Main Author: Abdullah, Noradilin
• Format: Thesis
• Published: 2013
• Subjects: Q Science (General); QH Natural history
• Online Access: http://studentsrepo.um.edu.my/4558/1/1_%2D_Cover_page.pdf; http://studentsrepo.um.edu.my/4558/2/2_%2D_Title_page.pdf; http://studentsrepo.um.edu.my/4558/3/3_%2D_Abstract%2C_List_of_Figures_and_Tables%2C_Acknowledgement.pdf; http://studentsrepo.um.edu.my/4558/4/4_%2D_Table_of_contents.pdf; http://studentsrepo.um.edu.my/4558/5/5_%2D_Thesis_Chapter_1%2D7.pdf; http://studentsrepo.um.edu.my/4558/

ST332 is a 332 bp gene marker used in a patented Salmonella kit for detection of foodborne pathogen, Salmonella Typhi. As the gene is specific in DNA based detection by using polymerase chain reaction (PCR), its specificity in immunoassay is studied. For this purpose, an expression system of the gen...