Acid denaturation of aspergillus niger glucoamylase and structural transitions induced by various alcohols and polyols / Mohammed Suleiman Mohammed Zaroog

• Main Author: Zaroog, Mohammed Suleiman Mohammed
• Format: Thesis
• Published: 2013
• Subjects: Q Science (General); QH Natural history
• Online Access: http://studentsrepo.um.edu.my/4245/11/Front_page.pdf; http://studentsrepo.um.edu.my/4245/14/Title_page.pdf; http://studentsrepo.um.edu.my/4245/7/Declaration_page.pdf; http://studentsrepo.um.edu.my/4245/1/BIOGRAPHY.pdf; http://studentsrepo.um.edu.my/4245/12/Initial_pages.pdf; http://studentsrepo.um.edu.my/4245/13/Thesis.pdf; http://studentsrepo.um.edu.my/4245/8/Figures_of_Literature_Review.pdf; http://studentsrepo.um.edu.my/4245/9/Figures_of_Materials_and_Methods.pdf; http://studentsrepo.um.edu.my/4245/10/Figures_of_Results.pdf; http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f986$002fSD_ILS:986532/one?qu=Acid+denaturation+of+aspergillus+niger; http://studentsrepo.um.edu.my/4245/

Acid denaturation of Aspergillus niger glucoamylase was studied using different conformational probes. Both far-UV CD spectral signal (MRE222nm) and tryptophan fluorescence remained unchanged in the pH range, 7.0–3.0 but decreased significantly below pH 3.0, whereas the ANS fluorescence showed a mar...