Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz
Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to init...
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my.um.stud.35702013-07-10T03:59:59Z Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz Aziz, Habsah QH301 Biology TP Chemical technology Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study the correlation between the day 1 post-apheresis and day 2 pre-apheresis CD34+ count and to study the collection efficiency of the apheresis protocol used. Forty eight patients with a total of 94 PBSC harvesting procedures were analyzed. All apheresis were performed using a COBE Spectra TM Apheresis System. Pre and post apheresis peripheral blood CD34+ cells as well as the harvested CD34+ cell count were analysed using FACSCalibur flow cytometer. The CD34+ harvesting cut-off value was determined using the Receiver Operating Characteristic (ROC) curve analysis and the correlation study was done using Pearson’s Correlation Analysis. The cut-off value of 27 cells/ul has been shown to predict a stem cell yield of 2 x106 CD34+ cells/ kg body weight with a single apheresis with 95% sensitivity and 82.4% specificity. Pre-apheresis peripheral blood CD34+ cell count correlated well with the stem cell yields (r = 0.963 and p<0.001). The day 1 post-apheresis peripheral blood CD34+ cell count correlates well with day 2 pre-apheresis count (r = 0.895), and a value of ≥ 25 cells/ul and may be able to predict the day 2 stem cell yield of 2 x106 CD34+ cells/ kg body weight. The median overall collection efficiency of the stem cell harvesting protocol was 63.7%. In conclusion, this study has shown that PB CD34+ count of 27 cells/ul will predict the stem cell yields of 2 x106 CD34+ cells/ kg body weight, and the apheresis protocol used for peripheral blood stem cell harvesting has gave satisfactory collection efficiency. 2010 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3570/2/Habsah_SGF40020_Prelims.pdf application/pdf http://studentsrepo.um.edu.my/3570/1/Habsah_SGF040020.pdf http://www.pendeta.um.edu.my/uhtbin/cgisirsi/x/0/0/57/5/3?searchdata1=856497{CKEY}&searchfield1=GENERAL^SUBJECT^GENERAL^^&user_id=WEBSERVER Aziz, Habsah (2010) Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3570/ |
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QH301 Biology TP Chemical technology Aziz, Habsah Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
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Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study the correlation between the day 1 post-apheresis and day 2 pre-apheresis CD34+ count and to study the collection efficiency of the apheresis protocol used. Forty eight patients with a total of 94 PBSC harvesting procedures were analyzed. All apheresis were performed using a COBE Spectra TM Apheresis System. Pre and post apheresis peripheral blood CD34+ cells as well as the harvested CD34+ cell count were analysed using FACSCalibur flow cytometer. The CD34+ harvesting cut-off value was determined using the Receiver Operating Characteristic (ROC) curve analysis and the correlation study was done using Pearson’s Correlation Analysis. The cut-off value of 27 cells/ul has been shown to predict a stem cell yield of 2 x106 CD34+ cells/ kg body weight with a single apheresis with 95% sensitivity and 82.4% specificity. Pre-apheresis peripheral blood CD34+ cell count correlated well with the stem cell yields (r = 0.963 and p<0.001). The day 1 post-apheresis peripheral blood CD34+ cell count correlates well with day 2 pre-apheresis count (r = 0.895), and a value of ≥ 25 cells/ul and may be able to predict the day 2 stem cell yield of 2 x106 CD34+ cells/ kg body weight. The median overall collection efficiency of the stem cell harvesting protocol was 63.7%.
In conclusion, this study has shown that PB CD34+ count of 27 cells/ul will predict the stem cell yields of 2 x106 CD34+ cells/ kg body weight, and the apheresis protocol used for peripheral blood stem cell harvesting has gave satisfactory collection efficiency. |
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Thesis |
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Aziz, Habsah |
author_facet |
Aziz, Habsah |
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Aziz, Habsah |
title |
Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
title_short |
Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
title_full |
Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
title_fullStr |
Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
title_full_unstemmed |
Estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood CD34[+] cells quantification / Habsah Aziz |
title_sort |
estimation of the product yield and collection efficiency of peripheral blood stem cell harvesting using peripheral blood cd34[+] cells quantification / habsah aziz |
publishDate |
2010 |
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http://studentsrepo.um.edu.my/3570/2/Habsah_SGF40020_Prelims.pdf http://studentsrepo.um.edu.my/3570/1/Habsah_SGF040020.pdf http://www.pendeta.um.edu.my/uhtbin/cgisirsi/x/0/0/57/5/3?searchdata1=856497{CKEY}&searchfield1=GENERAL^SUBJECT^GENERAL^^&user_id=WEBSERVER http://studentsrepo.um.edu.my/3570/ |
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