An in vitro study to compare coatings of peptide and collagen on hydroxyapatite coated titanium surfaces / Mohsin Nazir
Introduction: There is a paradigm shift in implantology in recent times. From implants being classically inert, and unable to interact with their environment, to them being biomimetic with the ability to affect their surroundings. Implants and more importantly, their surfaces, nowadays are design...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/10414/4/mohsin.pdf http://studentsrepo.um.edu.my/10414/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: There is a paradigm shift in implantology in recent times. From implants being
classically inert, and unable to interact with their environment, to them being biomimetic with the
ability to affect their surroundings. Implants and more importantly, their surfaces, nowadays are
designed to mimic nature. Therefore, the aim of this study was to compare the cell morphology,
proliferation and differentiation of hydroxyapatite (HA) coated titanium surfaces biofunctionalized
with RGD, and Collagen. Objectives: The first objective of the study was to modify the surface of
the implant by gritting, sandblasting, and acid-etching the CPTi surfaces so that they are comparable
to the commercially available implant surfaces. The second objective was to coat the modified
surfaces with HA using a Simulated Body Fluid (SBF) solution so that a uniform layer of HA is
formed on top of the samples. The third objective was to use physical adsorption for functionalizing
RGD and Collagen on the HA/Tricalcium Phospahte coated CPTi surfaces. The final objective was
to use the HA/Tricalcium Phosphate as the control to compare the in vitro response on the samples
using human osteoblasts. Methods: There were three phases to this experiment, namely, the surface
modification phase where gritting, sandblasting, and acid etching took place. The surface
characterization phase, where the CPTi samples were coated with hydroxyapatite (HA) with
simulated body fluid (SBF), RGD and collagen. The last phase was the in vitro phase where cell
morphology, cell viability, cell proliferation and mineralization were assessed using human osteoblast
cells (HOB) for the three different groups of surface coatings. Results and Discussion: The
sandblasted samples showed an average roughness (Sa) value of 2.48 ± 0.74µm. The acid-etched
samples displayed a lesser roughness than their sandblasted counterparts, that is, an average Sa of
2.35 ± 0.18µm. Within the HA/Tricalcium phosphate coated samples, there was a general increase in
|
|---|