Gastroprotective mechanism of Bauhinia thonningii Schum

Ethnopharmacological relevance: Bauhinia thonningii Schum. (Cesalpiniaceae) is locally known as Tambarib and used to treat various diseases including gastric ulcer. Aim of the study: The current study aims to evaluate the gastroprotecive mechanism(s) of methanolic (MEBT) and chloroform (CEBT) extrac...

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Main Authors: Abdelwahab, S.I., Taha, M.M.E., Abdulla, M.A., Nordin, N., Hadi, A.H.A., Mohan, S., Jayapalan, J.J., Hashim, Onn Haji
Format: Article
Language:English
Published: 2013
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Online Access:http://eprints.um.edu.my/9847/1/Abdelwahab-2013-Gastroprotective_mec.pdf
http://eprints.um.edu.my/9847/
http://www.sciencedirect.com/science/article/pii/S0378874113002924
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spelling my.um.eprints.98472019-10-24T08:28:50Z http://eprints.um.edu.my/9847/ Gastroprotective mechanism of Bauhinia thonningii Schum Abdelwahab, S.I. Taha, M.M.E. Abdulla, M.A. Nordin, N. Hadi, A.H.A. Mohan, S. Jayapalan, J.J. Hashim, Onn Haji R Medicine Ethnopharmacological relevance: Bauhinia thonningii Schum. (Cesalpiniaceae) is locally known as Tambarib and used to treat various diseases including gastric ulcer. Aim of the study: The current study aims to evaluate the gastroprotecive mechanism(s) of methanolic (MEBT) and chloroform (CEBT) extracts of Bauhinia thonningii leaves on ethanol-induced gastric ulceration. Materials and methods: Gastric acidity, quantification and histochemistry of mucus, gross and microscopic examination, nitric oxide, lipid peroxidation, 2D gel electrophoresis, mass spectroscopy and biochemical tests were utilized to assess the mechanism(s) underlying the gastroprotective effects of MEBT and CEBT. Effect of these extracts into lipopolysaccharide/interferon-gamma stimulated rodent cells were done in vitro. In vitro and in vivo toxicity studies were also conducted. Antioxidant activities of MEBT and CEBT were examined using DPPH, FRAP and ORAC assays. Phytochemical analyses of MEBT and CEBT were conducted using chemical and spectroscopic methods. Results: Gross and histological features confirmed the anti-ulcerogenic properties of Bauhinia thonningii. Gastroprotective mechanism of MEBT was observed to be mediated through the modulation of PAS-reactive substances, MDA and proteomics biomarkers (creatine kinase, malate dehydrogenase, ATP synthase, actin and thioredoxin). MEBT and CEBT showed no significant in vitro and in vivo effects on nitric oxide. Methanolic extract (MEBT) showed superior gastroprotective effects, polyphenolic content and antioxidant activities compared to CEBT. The plant extracts showed no in vitro or in vivo toxicity. Conclusion: It could be concluded that MEBT possesses anti-ulcer activity, which could be attributed to the inhibition of ethanol-induced oxidative damage and the intervention in proteomic pathways but not the nitric oxide pathway. (C) 2013 Elsevier Ireland Ltd. All rights reserved. 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9847/1/Abdelwahab-2013-Gastroprotective_mec.pdf Abdelwahab, S.I. and Taha, M.M.E. and Abdulla, M.A. and Nordin, N. and Hadi, A.H.A. and Mohan, S. and Jayapalan, J.J. and Hashim, Onn Haji (2013) Gastroprotective mechanism of Bauhinia thonningii Schum. Journal of Ethnopharmacology, 148 (1). pp. 277-286. ISSN 0378-8741 http://www.sciencedirect.com/science/article/pii/S0378874113002924 10.1016/j.jep.2013.04.027
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Abdelwahab, S.I.
Taha, M.M.E.
Abdulla, M.A.
Nordin, N.
Hadi, A.H.A.
Mohan, S.
Jayapalan, J.J.
Hashim, Onn Haji
Gastroprotective mechanism of Bauhinia thonningii Schum
description Ethnopharmacological relevance: Bauhinia thonningii Schum. (Cesalpiniaceae) is locally known as Tambarib and used to treat various diseases including gastric ulcer. Aim of the study: The current study aims to evaluate the gastroprotecive mechanism(s) of methanolic (MEBT) and chloroform (CEBT) extracts of Bauhinia thonningii leaves on ethanol-induced gastric ulceration. Materials and methods: Gastric acidity, quantification and histochemistry of mucus, gross and microscopic examination, nitric oxide, lipid peroxidation, 2D gel electrophoresis, mass spectroscopy and biochemical tests were utilized to assess the mechanism(s) underlying the gastroprotective effects of MEBT and CEBT. Effect of these extracts into lipopolysaccharide/interferon-gamma stimulated rodent cells were done in vitro. In vitro and in vivo toxicity studies were also conducted. Antioxidant activities of MEBT and CEBT were examined using DPPH, FRAP and ORAC assays. Phytochemical analyses of MEBT and CEBT were conducted using chemical and spectroscopic methods. Results: Gross and histological features confirmed the anti-ulcerogenic properties of Bauhinia thonningii. Gastroprotective mechanism of MEBT was observed to be mediated through the modulation of PAS-reactive substances, MDA and proteomics biomarkers (creatine kinase, malate dehydrogenase, ATP synthase, actin and thioredoxin). MEBT and CEBT showed no significant in vitro and in vivo effects on nitric oxide. Methanolic extract (MEBT) showed superior gastroprotective effects, polyphenolic content and antioxidant activities compared to CEBT. The plant extracts showed no in vitro or in vivo toxicity. Conclusion: It could be concluded that MEBT possesses anti-ulcer activity, which could be attributed to the inhibition of ethanol-induced oxidative damage and the intervention in proteomic pathways but not the nitric oxide pathway. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
format Article
author Abdelwahab, S.I.
Taha, M.M.E.
Abdulla, M.A.
Nordin, N.
Hadi, A.H.A.
Mohan, S.
Jayapalan, J.J.
Hashim, Onn Haji
author_facet Abdelwahab, S.I.
Taha, M.M.E.
Abdulla, M.A.
Nordin, N.
Hadi, A.H.A.
Mohan, S.
Jayapalan, J.J.
Hashim, Onn Haji
author_sort Abdelwahab, S.I.
title Gastroprotective mechanism of Bauhinia thonningii Schum
title_short Gastroprotective mechanism of Bauhinia thonningii Schum
title_full Gastroprotective mechanism of Bauhinia thonningii Schum
title_fullStr Gastroprotective mechanism of Bauhinia thonningii Schum
title_full_unstemmed Gastroprotective mechanism of Bauhinia thonningii Schum
title_sort gastroprotective mechanism of bauhinia thonningii schum
publishDate 2013
url http://eprints.um.edu.my/9847/1/Abdelwahab-2013-Gastroprotective_mec.pdf
http://eprints.um.edu.my/9847/
http://www.sciencedirect.com/science/article/pii/S0378874113002924
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score 13.154949