Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design

• Main Authors: Jamil, Z., Mohamad Annuar, M.S., Ibrahim, S., Vikineswary, S.
• Format: Article
• Language: English
• Published: 2009
• Subjects: T Technology (General); TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.um.edu.my/9433/1/Optimization_of_phototrophic_hydrogen_production_by_Rhodopseudomonas_palustris_PBUM001_via_statistical_experimental_design.pdf; http://eprints.um.edu.my/9433/; http://www.scopus.com/inward/record.url?eid=2-s2.0-68349137950&partnerID=40&md5=d8eae97960ff05500c40d640ea5d5d81 http://www.sciencedirect.com/science/article/pii/S0360319909008441 http://ac.els-cdn.com/S0360319909008441/1-s2.0-S0360319909008441-main.pdf?t

Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100 (v/v) POME concentration, 10 (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71 respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85. © 2009 International Association for Hydrogen Energy.