Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes
Physical manipulation of polarizable particles generated by the transient force when placed in non-uniform electrical fields is termed dielectrophoresis. The electrophysiological make-up of the particle and the surrounding medium decides the magnitude of the force; hence specific DEP profile may be...
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2012
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my.um.eprints.93142021-01-20T07:50:36Z http://eprints.um.edu.my/9314/ Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes Kadri, Nahrizul Adib Razak, Mohd Azhar Mohd Ibrahim, Fatimah R Medicine T Technology (General) TA Engineering (General). Civil engineering (General) Physical manipulation of polarizable particles generated by the transient force when placed in non-uniform electrical fields is termed dielectrophoresis. The electrophysiological make-up of the particle and the surrounding medium decides the magnitude of the force; hence specific DEP profile may be acquired for any polarizable particles based on the electrical properties of the membrane and cytoplasm alone. Any changes to these parameters may be detected, in turn, by observing the corresponding DEP spectra. The reason DEP is not applied widely in spite of its non-invasiveness primarily is that the process is time consuming. The proposed DEP-based lab-on-chip application of semi-automated cell electrophysiology characterization allowed concurrent DEP experiments to be conducted serially, which significantly reduced the required experiment time. This capability is achieved via the employment of a planar microelectrode with modified dot microarray geometry. The results showed that the system is capable of producing a complete DEP spectrum profile for K562 leukemic cells between the 1kHz to 1MHz frequency range in less than 10 minutes. © 2012 by ESG. Electrochemical Science Group 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9314/1/Near_real-time_electrochemical_analysis_of_carcinogenic_cell_lines_using_planar_microelectrodes.pdf Kadri, Nahrizul Adib and Razak, Mohd Azhar Mohd and Ibrahim, Fatimah (2012) Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes. International Journal of Electrochemical Science, 7 (6). pp. 5633-5642. ISSN 1452-3981 http://www.electrochemsci.org/papers/vol7/7087750.pdf |
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R Medicine T Technology (General) TA Engineering (General). Civil engineering (General) Kadri, Nahrizul Adib Razak, Mohd Azhar Mohd Ibrahim, Fatimah Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
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Physical manipulation of polarizable particles generated by the transient force when placed in non-uniform electrical fields is termed dielectrophoresis. The electrophysiological make-up of the particle and the surrounding medium decides the magnitude of the force; hence specific DEP profile may be acquired for any polarizable particles based on the electrical properties of the membrane and cytoplasm alone. Any changes to these parameters may be detected, in turn, by observing the corresponding DEP spectra. The reason DEP is not applied widely in spite of its non-invasiveness primarily is that the process is time consuming. The proposed DEP-based lab-on-chip application of semi-automated cell electrophysiology characterization allowed concurrent DEP experiments to be conducted serially, which significantly reduced the required experiment time. This capability is achieved via the employment of a planar microelectrode with modified dot microarray geometry. The results showed that the system is capable of producing a complete DEP spectrum profile for K562 leukemic cells between the 1kHz to 1MHz frequency range in less than 10 minutes. © 2012 by ESG. |
format |
Article |
author |
Kadri, Nahrizul Adib Razak, Mohd Azhar Mohd Ibrahim, Fatimah |
author_facet |
Kadri, Nahrizul Adib Razak, Mohd Azhar Mohd Ibrahim, Fatimah |
author_sort |
Kadri, Nahrizul Adib |
title |
Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
title_short |
Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
title_full |
Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
title_fullStr |
Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
title_full_unstemmed |
Near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
title_sort |
near real-time electrochemical analysis of carcinogenic cell lines using planar microelectrodes |
publisher |
Electrochemical Science Group |
publishDate |
2012 |
url |
http://eprints.um.edu.my/9314/1/Near_real-time_electrochemical_analysis_of_carcinogenic_cell_lines_using_planar_microelectrodes.pdf http://eprints.um.edu.my/9314/ http://www.electrochemsci.org/papers/vol7/7087750.pdf |
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