Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia
The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR)poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MD...
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my.um.eprints.90332018-10-15T04:19:59Z http://eprints.um.edu.my/9033/ Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia Lim, K.T. Yasin, R. Yeo, C.C. Puthucheary, S.D. Thong, Kwai Lin QR Microbiology The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR)poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E. coli harbored the blaTEM gene. Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M. Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron encoded intI1 integrase being the majority. Amplification and sequence analysis of the 5�CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E. coli isolates. Hindawi Publishing Corporation 2009-06-11 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9033/1/Lim_et_al.%2C_2009_%283%29.pdf Lim, K.T. and Yasin, R. and Yeo, C.C. and Puthucheary, S.D. and Thong, Kwai Lin (2009) Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. Journal of Biomedicine and Biotechnology, 2009 (165637). pp. 1-10. ISSN 1110-7243 |
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The emergence of Escherichia coli that produce extended spectrum β-lactamases (ESBLs) and are multidrug resistant (MDR)poses antibiotic management problems. Forty-seven E. coli isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene-specific primers showed that 87.5% of the ESBL-producing E. coli harbored the blaTEM gene. Other ESBL-encoding genes detected were blaOXA, blaSHV, and blaCTX-M. Integron-encoded integrases were detected in 55.3% of isolates, with class 1 integron encoded intI1 integrase being the majority. Amplification and sequence analysis of the 5�CS region of the integrons showed known antibiotic resistance-encoding gene cassettes of various sizes that were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the antibiotic resistance genes were likely plasmid-encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR-based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and enterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and REP-PCR methods were more discriminative than RAPD in subtyping the E. coli isolates. |
format |
Article |
author |
Lim, K.T. Yasin, R. Yeo, C.C. Puthucheary, S.D. Thong, Kwai Lin |
author_facet |
Lim, K.T. Yasin, R. Yeo, C.C. Puthucheary, S.D. Thong, Kwai Lin |
author_sort |
Lim, K.T. |
title |
Characterization of Multidrug Resistant ESBL-Producing
Escherichia coli Isolates from Hospitals in Malaysia |
title_short |
Characterization of Multidrug Resistant ESBL-Producing
Escherichia coli Isolates from Hospitals in Malaysia |
title_full |
Characterization of Multidrug Resistant ESBL-Producing
Escherichia coli Isolates from Hospitals in Malaysia |
title_fullStr |
Characterization of Multidrug Resistant ESBL-Producing
Escherichia coli Isolates from Hospitals in Malaysia |
title_full_unstemmed |
Characterization of Multidrug Resistant ESBL-Producing
Escherichia coli Isolates from Hospitals in Malaysia |
title_sort |
characterization of multidrug resistant esbl-producing
escherichia coli isolates from hospitals in malaysia |
publisher |
Hindawi Publishing Corporation |
publishDate |
2009 |
url |
http://eprints.um.edu.my/9033/1/Lim_et_al.%2C_2009_%283%29.pdf http://eprints.um.edu.my/9033/ |
_version_ |
1643688450237399040 |
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13.160551 |