Novel antiviral activity of baicalein against dengue virus
Background: Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants ar...
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my.um.eprints.82512019-11-18T09:01:32Z http://eprints.um.edu.my/8251/ Novel antiviral activity of baicalein against dengue virus Zandi, Keivan Teoh, Boon Teong Sam, Sing Sin Wong, Pooi Fong Mustafa, Mohd Rais AbuBakar, Sazaly R Medicine Background: Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits. Methods: In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR. Results: Baicalein inhibited DENV-2 replication in Vero cells with IC50= 6.46 μg/mL and SI= 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 μg/mL and SI= 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC 50= 1.55 μg/mL and showed anti-adsorption effect with IC50 = 7.14 μg/mL. Conclusions: Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV. BioMed Central 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8251/1/1472-6882-12-214.pdf Zandi, Keivan and Teoh, Boon Teong and Sam, Sing Sin and Wong, Pooi Fong and Mustafa, Mohd Rais and AbuBakar, Sazaly (2012) Novel antiviral activity of baicalein against dengue virus. BMC Complementary and Alternative Medicine, 12 (1). pp. 1-9. ISSN 1472-6882 https://doi.org/10.1186/1472-6882-12-214 doi:10.1186/1472-6882-12-214 |
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R Medicine Zandi, Keivan Teoh, Boon Teong Sam, Sing Sin Wong, Pooi Fong Mustafa, Mohd Rais AbuBakar, Sazaly Novel antiviral activity of baicalein against dengue virus |
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Background: Dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits. Methods: In the present study, antiviral activity of a bioflavonoid, baicalein, was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cells using focus forming unit reduction assay and quantitative RT-PCR. Results: Baicalein inhibited DENV-2 replication in Vero cells with IC50= 6.46 μg/mL and SI= 17.8 when added after adsorption to the cells. The IC50 against DENV-2 was 5.39 μg/mL and SI= 21.3 when cells were treated 5 hours before virus infection and continuously up to 4 days post infection. Baicalein exhibited direct virucidal effect against DENV-2 with IC 50= 1.55 μg/mL and showed anti-adsorption effect with IC50 = 7.14 μg/mL. Conclusions: Findings presented here suggest that baicalein exerts potent antiviral activity against DENV. Baicalein possesses direct virucidal activity against DENV besides its effects against dengue virus adsorption and intracellular replication of DENV-2. Baicalein, hence, should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV. |
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Article |
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Zandi, Keivan Teoh, Boon Teong Sam, Sing Sin Wong, Pooi Fong Mustafa, Mohd Rais AbuBakar, Sazaly |
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Zandi, Keivan Teoh, Boon Teong Sam, Sing Sin Wong, Pooi Fong Mustafa, Mohd Rais AbuBakar, Sazaly |
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Zandi, Keivan |
title |
Novel antiviral activity of baicalein against dengue virus |
title_short |
Novel antiviral activity of baicalein against dengue virus |
title_full |
Novel antiviral activity of baicalein against dengue virus |
title_fullStr |
Novel antiviral activity of baicalein against dengue virus |
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Novel antiviral activity of baicalein against dengue virus |
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novel antiviral activity of baicalein against dengue virus |
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BioMed Central |
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2012 |
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http://eprints.um.edu.my/8251/1/1472-6882-12-214.pdf http://eprints.um.edu.my/8251/ https://doi.org/10.1186/1472-6882-12-214 |
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