Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin
The aims of this study were to investigate the combined effects of a natural compound 1²S-1²-acetoxychavicol acetate (ACA) with cisplatin (CDDP) on HPV-positive human cervical carcinoma cell lines (Ca Ski - low cisplatin sensitivity and HeLa - high cisplatin sensitivity), and to identify microRNAs (...
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my.um.eprints.77842019-03-01T09:22:47Z http://eprints.um.edu.my/7784/ Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin Phuah, N.H. In, L.L.A. Azmi, M.N. Ibrahim, H. Awang, Khalijah Nagoor, Noor Hasima QH301 Biology The aims of this study were to investigate the combined effects of a natural compound 1²S-1²-acetoxychavicol acetate (ACA) with cisplatin (CDDP) on HPV-positive human cervical carcinoma cell lines (Ca Ski - low cisplatin sensitivity and HeLa - high cisplatin sensitivity), and to identify microRNAs (miRNAs) modulated in response toward ACA and/or CDDP. It was revealed that both ACA and CDDP induced dose- and time-dependent cytotoxicity when used as a stand-alone agent, while synergistic effects were observed when used in combination with a combination index (CI) value of 0.74 ± 0.01 and 0.85 ± 0.01 in Ca Ski and HeLa cells, respectively. A total of 25 miRNAs were found to be significantly differentially expressed in response to ACA and/or CDDP. These include hsa-miR-138, hsa-miR-210, and hsa-miR-744 with predicted gene targets involved in signaling pathways regulating apoptosis and cell cycle progression. In conclusion, ACA acts as a chemosensitizer which synergistically potentiates the cytotoxic effect of CDDP in cervical cancer cells. The altered miRNA expression upon administration of ACA and/or CDDP suggests that miRNAs play an important role in anticancer drug responses, which can be manipulated for therapeutic purposes. © The Author(s) 2012. 2013 Article PeerReviewed Phuah, N.H. and In, L.L.A. and Azmi, M.N. and Ibrahim, H. and Awang, Khalijah and Nagoor, Noor Hasima (2013) Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin. Reproductive Sciences, 20 (5). pp. 567-578. ISSN 1933-7191 10.1177/1933719112459220 |
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QH301 Biology Phuah, N.H. In, L.L.A. Azmi, M.N. Ibrahim, H. Awang, Khalijah Nagoor, Noor Hasima Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
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The aims of this study were to investigate the combined effects of a natural compound 1²S-1²-acetoxychavicol acetate (ACA) with cisplatin (CDDP) on HPV-positive human cervical carcinoma cell lines (Ca Ski - low cisplatin sensitivity and HeLa - high cisplatin sensitivity), and to identify microRNAs (miRNAs) modulated in response toward ACA and/or CDDP. It was revealed that both ACA and CDDP induced dose- and time-dependent cytotoxicity when used as a stand-alone agent, while synergistic effects were observed when used in combination with a combination index (CI) value of 0.74 ± 0.01 and 0.85 ± 0.01 in Ca Ski and HeLa cells, respectively. A total of 25 miRNAs were found to be significantly differentially expressed in response to ACA and/or CDDP. These include hsa-miR-138, hsa-miR-210, and hsa-miR-744 with predicted gene targets involved in signaling pathways regulating apoptosis and cell cycle progression. In conclusion, ACA acts as a chemosensitizer which synergistically potentiates the cytotoxic effect of CDDP in cervical cancer cells. The altered miRNA expression upon administration of ACA and/or CDDP suggests that miRNAs play an important role in anticancer drug responses, which can be manipulated for therapeutic purposes. © The Author(s) 2012. |
format |
Article |
author |
Phuah, N.H. In, L.L.A. Azmi, M.N. Ibrahim, H. Awang, Khalijah Nagoor, Noor Hasima |
author_facet |
Phuah, N.H. In, L.L.A. Azmi, M.N. Ibrahim, H. Awang, Khalijah Nagoor, Noor Hasima |
author_sort |
Phuah, N.H. |
title |
Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
title_short |
Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
title_full |
Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
title_fullStr |
Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
title_full_unstemmed |
Alterations of MicroRNA expression patterns in human cervical carcinoma cells (Ca Ski) toward 1â'S-1â'-acetoxychavicol acetate and cisplatin |
title_sort |
alterations of microrna expression patterns in human cervical carcinoma cells (ca ski) toward 1â's-1â'-acetoxychavicol acetate and cisplatin |
publishDate |
2013 |
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http://eprints.um.edu.my/7784/ |
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1643688127817056256 |
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13.188404 |