A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles
A high Tc SQUID system for biological molecules (DNA) detection is developed. This system is based on a hybridization process. Two strands in a DNA molecule are held together by hydrogen bonds between base pairs like a ladder. The two strands are referred to as being complementary each other. HPVB 3...
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Institute of Electrical and Electronics Engineers (IEEE)
2005
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my.um.eprints.74662017-07-08T03:56:27Z http://eprints.um.edu.my/7466/ A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles Tanaka, S. Katsura, S. Hatsukade, Y. Toriyabe, C. Kurita, H. Aspanut, Z. QC Physics A high Tc SQUID system for biological molecules (DNA) detection is developed. This system is based on a hybridization process. Two strands in a DNA molecule are held together by hydrogen bonds between base pairs like a ladder. The two strands are referred to as being complementary each other. HPVB 33 (Human Papillomavirus Probes 33) was prepared as a DNA. One strand (Sample DNA) was labeled with Fe3O4 ultra-small magnetic particles and the other (probe DNA) was anchored on a glass slide. Then they were hybridized each other on the slide. After washing the excess sample DNA, the hybridized DNA was evaluated in the presence of excitation ac field by high Tc SQUID. The signal was initially proportional to the concentration of the sample DNA and then saturated. It means that the hybridization occurred successfully between the sample DNA and the probe DNA. Institute of Electrical and Electronics Engineers (IEEE) 2005 Article PeerReviewed Tanaka, S. and Katsura, S. and Hatsukade, Y. and Toriyabe, C. and Kurita, H. and Aspanut, Z. (2005) A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles. IEEE Transactions on Applied Superconductivity, 15 (2). pp. 664-667. ISSN 1051-8223 http://www.scopus.com/inward/record.url?eid=2-s2.0-22044455141&partnerID=40&md5=02eaf9164884b8ecfa4b8b53998f72ab 10.1109/tasc.2005.849992 |
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QC Physics Tanaka, S. Katsura, S. Hatsukade, Y. Toriyabe, C. Kurita, H. Aspanut, Z. A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
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A high Tc SQUID system for biological molecules (DNA) detection is developed. This system is based on a hybridization process. Two strands in a DNA molecule are held together by hydrogen bonds between base pairs like a ladder. The two strands are referred to as being complementary each other. HPVB 33 (Human Papillomavirus Probes 33) was prepared as a DNA. One strand (Sample DNA) was labeled with Fe3O4 ultra-small magnetic particles and the other (probe DNA) was anchored on a glass slide. Then they were hybridized each other on the slide. After washing the excess sample DNA, the hybridized DNA was evaluated in the presence of excitation ac field by high Tc SQUID. The signal was initially proportional to the concentration of the sample DNA and then saturated. It means that the hybridization occurred successfully between the sample DNA and the probe DNA. |
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Article |
author |
Tanaka, S. Katsura, S. Hatsukade, Y. Toriyabe, C. Kurita, H. Aspanut, Z. |
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Tanaka, S. Katsura, S. Hatsukade, Y. Toriyabe, C. Kurita, H. Aspanut, Z. |
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Tanaka, S. |
title |
A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
title_short |
A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
title_full |
A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
title_fullStr |
A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
title_full_unstemmed |
A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles |
title_sort |
dna detection system based upon a high tcsquid and ultra-small magnetic particles |
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Institute of Electrical and Electronics Engineers (IEEE) |
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2005 |
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http://eprints.um.edu.my/7466/ http://www.scopus.com/inward/record.url?eid=2-s2.0-22044455141&partnerID=40&md5=02eaf9164884b8ecfa4b8b53998f72ab |
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13.160551 |