Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro

• Main Authors: Yusof, R., Clum, S., Wetzel, M., Murthy, H.M.K., Padmanabhan, R.
• Format: Article
• Language: English
• Published: 2000
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/7148/1/Yusof-2000-Purified_NS2B_NS3_se.pdf; http://eprints.um.edu.my/7148/

Dengue virus type 2 NS3, a multifunctional protein, has a serine protease domain (NS3pro) that requires the conserved hydrophilic domain of NS2B for protease activity in cleavage of the polyprotein precursor at sites following two basic amino acids. In this study, we report the expression of the NS2...