Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker

The elimination of antibiotic or herbicide resistance gene usage in genetically modified plants is being encouraged due to public concern. In response to this, alternative selection systems for the recovery of transgenic oil palm were developed using positive selectable markers. To establish a selec...

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Main Authors: Bahariah, B., Parveez, G.K.A., Khalid, N.
Format: Article
Language:English
Published: 2012
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Online Access:http://eprints.um.edu.my/5916/1/Determining_the_optimal_concentration_of_mannose_as_an_effective_selection_agent_for_transformed_oil_palm_cells_using.pdf
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spelling my.um.eprints.59162013-04-24T01:44:16Z http://eprints.um.edu.my/5916/ Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker Bahariah, B. Parveez, G.K.A. Khalid, N. QH301 Biology The elimination of antibiotic or herbicide resistance gene usage in genetically modified plants is being encouraged due to public concern. In response to this, alternative selection systems for the recovery of transgenic oil palm were developed using positive selectable markers. To establish a selection system that utilises the phosphomannose isomerase (pmi) gene for oil palm transformation, we first determined the optimal mannose concentration for selecting the transformed cells. Non-transformed embryogenic calli were cultured on media containing various combinations and concentrations of mannose and a usable source of carbon, i.e. sucrose, ranging in content from 0 to 30 g litre . Sucrose is often used as a carbon source in plant tissue culture media. The embryogenic calli were subcultured onto similar fresh media every four weeks, and growth was recorded monthly up to five months. From the 10 combinations of mannose and sucrose evaluated, mannose:sucrose at 30:0 g litre was shown to be the most effective for selection because at this concentration the least plant grozvth was demonstrated for non-transformed embryogenic calli. We will thereafter use this particular concentration of mannose to select for oil palm embryogenic calli transformed with the pmi gene using biolistic bombardment. 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5916/1/Determining_the_optimal_concentration_of_mannose_as_an_effective_selection_agent_for_transformed_oil_palm_cells_using.pdf Bahariah, B. and Parveez, G.K.A. and Khalid, N. (2012) Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker. Journal of Oil Palm Research, 24. pp. 1250-1259. ISSN 1511-2780
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Bahariah, B.
Parveez, G.K.A.
Khalid, N.
Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
description The elimination of antibiotic or herbicide resistance gene usage in genetically modified plants is being encouraged due to public concern. In response to this, alternative selection systems for the recovery of transgenic oil palm were developed using positive selectable markers. To establish a selection system that utilises the phosphomannose isomerase (pmi) gene for oil palm transformation, we first determined the optimal mannose concentration for selecting the transformed cells. Non-transformed embryogenic calli were cultured on media containing various combinations and concentrations of mannose and a usable source of carbon, i.e. sucrose, ranging in content from 0 to 30 g litre . Sucrose is often used as a carbon source in plant tissue culture media. The embryogenic calli were subcultured onto similar fresh media every four weeks, and growth was recorded monthly up to five months. From the 10 combinations of mannose and sucrose evaluated, mannose:sucrose at 30:0 g litre was shown to be the most effective for selection because at this concentration the least plant grozvth was demonstrated for non-transformed embryogenic calli. We will thereafter use this particular concentration of mannose to select for oil palm embryogenic calli transformed with the pmi gene using biolistic bombardment.
format Article
author Bahariah, B.
Parveez, G.K.A.
Khalid, N.
author_facet Bahariah, B.
Parveez, G.K.A.
Khalid, N.
author_sort Bahariah, B.
title Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
title_short Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
title_full Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
title_fullStr Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
title_full_unstemmed Determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
title_sort determining the optimal concentration of mannose as an effective selection agent for transformed oil palm cells using the phosphomannose isomerase (pmi) gene as a positive selectable marker
publishDate 2012
url http://eprints.um.edu.my/5916/1/Determining_the_optimal_concentration_of_mannose_as_an_effective_selection_agent_for_transformed_oil_palm_cells_using.pdf
http://eprints.um.edu.my/5916/
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score 13.222552