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Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers

Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin...

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Main Authors: Zainuddin, Z., Khalid, Norzulaani, Tan, C.S.
Format: Article
Language:English
Published: Faculty of Science, University of Malaya 2002
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf
http://eprints.um.edu.my/5899/
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spelling my.um.eprints.58992021-04-27T07:50:38Z http://eprints.um.edu.my/5899/ Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers Zainuddin, Z. Khalid, Norzulaani Tan, C.S. QH301 Biology Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues. Faculty of Science, University of Malaya 2002 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf Zainuddin, Z. and Khalid, Norzulaani and Tan, C.S. (2002) Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers. Malaysian Journal of Science, 21 (1 & 2). pp. 25-30. ISSN 1394-3065
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Zainuddin, Z.
Khalid, Norzulaani
Tan, C.S.
Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
description Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues.
format Article
author Zainuddin, Z.
Khalid, Norzulaani
Tan, C.S.
author_facet Zainuddin, Z.
Khalid, Norzulaani
Tan, C.S.
author_sort Zainuddin, Z.
title Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
title_short Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
title_full Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
title_fullStr Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
title_full_unstemmed Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
title_sort comparison between two transformation systems for introducing cmv coat protein construct into local hot peppers
publisher Faculty of Science, University of Malaya
publishDate 2002
url http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf
http://eprints.um.edu.my/5899/
_version_ 1698697303241523200
score 13.160551

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