Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002
Pulsed field gel electrophoresis (PFGE) and antimicrobial susceptibility analysis were undertaken on twenty-three strains of Salmonella enterica serovar Tshiongwe, an unusual serovar, which recently emerged in Malaysia. Antimicrobial susceptibility analysis showed that all the strains were sensitive...
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Seameo Regional Tropical Medicine & Public Health
2004
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my.um.eprints.56212018-10-15T02:33:27Z http://eprints.um.edu.my/5621/ Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 Thong, Kwai Lin Bakeri, Shamsilawani Ahmad Lai, Kin Seng Koh, Yin Tee Taib, Mohd Zainuldin Lim, V.K.E. Yasin, Rohani Md Q Science (General) QH Natural history QR Microbiology Pulsed field gel electrophoresis (PFGE) and antimicrobial susceptibility analysis were undertaken on twenty-three strains of Salmonella enterica serovar Tshiongwe, an unusual serovar, which recently emerged in Malaysia. Antimicrobial susceptibility analysis showed that all the strains were sensitive to ampicilin, chloramphenicol, cotrimoxazole, and kanamycin. Twenty (87) and 8 (3.5) strains had resistance to tetracycline and streptomycin respectively. PFGE analysis subtyped 23 strains into 10 profiles (Dice coefficient of similarity, F = 0.7-1.0). The predominant profile, X1 was found in both clinical and environmental isolates and was widely distributed in different parts of Malaysia during the study period. In addition, isolates recovered from food, a hand-towel, apron and the surface of a table-top in one particular location had unique, indistinguishable profiles (X4/4a) and identical antibiograms. Similarly, isolates from cooked meat and a chopping board had PFGE profiles similar to some human isolates. These probably indicated cross-contamination and poor hygiene in food practices, hence contributing to Salmonellosis. Factors causing the emergence of this rare Salmonella serovar being responsible for food poisoning episodes during the study period remained unclear. The study reiterated the usefulness and versatility of PFGE in the molecular subtyping of this rare Salmonella serovar in Malaysia. Seameo Regional Tropical Medicine & Public Health 2004 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5621/1/Research_note-_Molecular_subtyping_of_Salmonella_enterica_serovar_Tshiongwe_recently_isolated_in_Malaysia_during_2001-2002..pdf Thong, Kwai Lin and Bakeri, Shamsilawani Ahmad and Lai, Kin Seng and Koh, Yin Tee and Taib, Mohd Zainuldin and Lim, V.K.E. and Yasin, Rohani Md (2004) Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002. Southeast Asian Journal of Tropical Medicine and Public Health, 35 (1). pp. 92-96. ISSN 0125-1562 http://www.tm.mahidol.ac.th/seameo/2004_35_1/13-3180.pdf |
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Q Science (General) QH Natural history QR Microbiology Thong, Kwai Lin Bakeri, Shamsilawani Ahmad Lai, Kin Seng Koh, Yin Tee Taib, Mohd Zainuldin Lim, V.K.E. Yasin, Rohani Md Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
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Pulsed field gel electrophoresis (PFGE) and antimicrobial susceptibility analysis were undertaken on twenty-three strains of Salmonella enterica serovar Tshiongwe, an unusual serovar, which recently emerged in Malaysia. Antimicrobial susceptibility analysis showed that all the strains were sensitive to ampicilin, chloramphenicol, cotrimoxazole, and kanamycin. Twenty (87) and 8 (3.5) strains had resistance to tetracycline and streptomycin respectively. PFGE analysis subtyped 23 strains into 10 profiles (Dice coefficient of similarity, F = 0.7-1.0). The predominant profile, X1 was found in both clinical and environmental isolates and was widely distributed in different parts of Malaysia during the study period. In addition, isolates recovered from food, a hand-towel, apron and the surface of a table-top in one particular location had unique, indistinguishable profiles (X4/4a) and identical antibiograms. Similarly, isolates from cooked meat and a chopping board had PFGE profiles similar to some human isolates. These probably indicated cross-contamination and poor hygiene in food practices, hence contributing to Salmonellosis. Factors causing the emergence of this rare Salmonella serovar being responsible for food poisoning episodes during the study period remained unclear. The study reiterated the usefulness and versatility of PFGE in the molecular subtyping of this rare Salmonella serovar in Malaysia. |
format |
Article |
author |
Thong, Kwai Lin Bakeri, Shamsilawani Ahmad Lai, Kin Seng Koh, Yin Tee Taib, Mohd Zainuldin Lim, V.K.E. Yasin, Rohani Md |
author_facet |
Thong, Kwai Lin Bakeri, Shamsilawani Ahmad Lai, Kin Seng Koh, Yin Tee Taib, Mohd Zainuldin Lim, V.K.E. Yasin, Rohani Md |
author_sort |
Thong, Kwai Lin |
title |
Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
title_short |
Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
title_full |
Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
title_fullStr |
Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
title_full_unstemmed |
Research Note: Molecular Subtyping of Salmonella enterica serovar Tshiongwe Recently Isolated in Malaysia During 2001-2002 |
title_sort |
research note: molecular subtyping of salmonella enterica serovar tshiongwe recently isolated in malaysia during 2001-2002 |
publisher |
Seameo Regional Tropical Medicine & Public Health |
publishDate |
2004 |
url |
http://eprints.um.edu.my/5621/1/Research_note-_Molecular_subtyping_of_Salmonella_enterica_serovar_Tshiongwe_recently_isolated_in_Malaysia_during_2001-2002..pdf http://eprints.um.edu.my/5621/ http://www.tm.mahidol.ac.th/seameo/2004_35_1/13-3180.pdf |
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