Synthesis of 2 '-(5-Chloro-2-Hydroxybenzylidene) benzenesulfanohydrazide schiff base and its anti-ulcer activity in ethanol-induced gastric mucosal lesions in rats

• Main Authors: Gwaram, N.S., Abdulla, M.A., Ali, Hapipah Mohd, Musalam, L.
• Format: Article
• Published: Pharmacotherapy Group, University of Benin, Benin City 2012
• Subjects: QD Chemistry
• Online Access: http://eprints.um.edu.my/5144/; http://www.ajol.info/index.php/tjpr/article/viewFile/75882/66385

Purpose: To study the anti-ulcer activity of 2'-(5-Chloro-2-hydroxybenzylidene) benzenesulfanohydrazide Schiff base against ethanol-induced gastric mucosal lesions in rats. Methods: 2'-(5-Chloro-2-hydroxybenzylidene) benzenesulfano-hydrazide (Cl-BzSO-HAP) was synthesized, and structurally characterized by elemental analysis, FT-IR, H-1-NMR and C-13-NMR. Its preventive activity against ulcer induced by absolute ethanol in Sprague-Dawley rats were studied in vivo. Twenty four Sprague Dawley (SD) rats (12 males and 12 females) were assigned equally into 4 groups (n = 6), including negative control and positive control groups. The third group received a low oral dose of the compound (50 mg/kg) while the fourth group was administered a high oral dose (100 mg/kg). The degree of gastric lesion formation was assessed. Prior to dosing, the animals were fasted overnight. Results: Cl-BzSO-HAP showed significant preventive activity against ulcer induced by absolute ethanol. Oral administration of the compound (both low and high doses) prior to ethanol administration inhibited gastric lesion formation by 88.0 and 94.9%, respectively, compared to 82.7% for the positive control, cimetidine. Conclusion: Extensive gastric necrosis in rats induced by absolute ethanol was prevented by administration of Cl-BzSO-HAP resulting in reduced or total absence of lesions.