Enhancement of methane oxidation with effective methanotrophic mixed cultures

• Main Authors: Barasarathi, J., Agamuthu, Pariatamby
• Format: Article
• Published: Faculty of Science, University of Malaya 2011
• Subjects: QH301 Biology
• Online Access: http://eprints.um.edu.my/4972/; http://www.scopus.com/inward/record.url?eid=2-s2.0-79958704581&partnerID=40&md5=91addd6f87dd4240131d9b611564fd90

The emission of CH 4 from landfill is ranked third among the anthropogenic CH 4 sources and ranged between 19-40Tg/yr. The Microbial oxidation of landfill methane plays a significant role in reducing the emissions to the atmosphere. This study was carried out to the investigate the impact of several parameters on methane(CH 4) oxidation, using compost as biocover. Addition of dedicated methanotrophic bacterial cultures was also included.. Experiments with different concentrations of methanotrophic mixed cultures ranging from 2.33× 10 7CFU/g to 11.33× 10 77 CFU/g showed that the highest oxidation rate with addition of 5.33 × 10 7 CFU/g was 4.166 × 10 3ugg -1h -1. Experiments with different incubation temperature showed that highest oxidation rate of 4.166 × 10 3ugg -1h -1 was at 35°C Similar oxidation rates were obtained with the addition of mixed culture at 60% moisture content. The highest bacterial count was obtained at 35°C at 12.33 × 10 7 CFU/g while lowest was at 45°C The moisture at 60% showed the highest bacterial count at 10.66 × 10 7 CFU/g whereas 30% moisture showed the lowest count at 3 × 10 7 CFU/g. From this study we concluded that the addition of methanotrophic mixed culture gave a significant increase in methane oxidation compared to the control at the optimal temperature and moisture content.