The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study

BACKGROUND: Commercially available oral rinses contain active ingredients with concentration that is claimed by manufacturers to be effective as antiplaque agent. To date there has been no mention of the effect of oral rinse on the adherence of early plaque colonizers in plaque formation and the con...

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Main Authors: Zeeshan, Tayyaba, Qamar, Zeeshan, Abdul, Nishath Sayed, Soman, Cristalle, Bamousa, Badr, Marrapodi, Maria Maddalena, Cicciu, Marco, Minervini, Giuseppe
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Published: IOS Press 2024
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Online Access:http://eprints.um.edu.my/47133/
https://doi.org/10.3233/THC-231814
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spelling my.um.eprints.471332024-11-28T05:21:50Z http://eprints.um.edu.my/47133/ The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study Zeeshan, Tayyaba Qamar, Zeeshan Abdul, Nishath Sayed Soman, Cristalle Bamousa, Badr Marrapodi, Maria Maddalena Cicciu, Marco Minervini, Giuseppe RK Dentistry BACKGROUND: Commercially available oral rinses contain active ingredients with concentration that is claimed by manufacturers to be effective as antiplaque agent. To date there has been no mention of the effect of oral rinse on the adherence of early plaque colonizers in plaque formation and the concentration to be used before/after meals. OBJECTIVE: The chief aim of the study was to evaluate microbial retention on the salivary pellicle on treatment with oral rinses (CHX & EO)/PS (mimicking after meals use of mouth wash/PS). METHODS: Noordini's Artifical Mouth model was used for developing the single species biofilm with early microbial colonizers of oral biofilm (A. viscosus, Strep. mitis and Strep. sanguinis respectively). The microbial retention on use of oral rinses comprising of CHX and EO as an active ingredients respectively was compared with Curcumin PS. For evaluating the microbial retention, the pellicle with microbial inoculation was developed on the glass beads in the mouth model. Subsequently the respective single specie biofilm was exposed to the mouth wash and PS after inoculation. It mimicked as use of mouth wash/PS after meals. The bacterial count in the dental biofilm was evaluated on serial dilution (CFU/ml). Sterile deionized water was used as a negative control. For qualitative analysis, Scanning electron microscope (SEM) was used to evaluate the microbial count. RESULTS: From the data it was observed that for the treatment of single species experimental biofilm with commercially available mouth rinses (CHX & EO) and PS (curcumin), there was significant retention for S.mitis, S.sanguinis and A.viscosus. There was no significant difference observed between PS and CHX treated single species biofilm. Whereas a significant difference was observed between EO treated biofilms and CHX/PS treated biofilms (p <= 0.05). CONCLUSION: It can be concluded from the results that curcumin PS and CHX should not be used after meals whereas EO containing mouth rinse can be used to maintain the oral mocroflora. IOS Press 2024 Article PeerReviewed Zeeshan, Tayyaba and Qamar, Zeeshan and Abdul, Nishath Sayed and Soman, Cristalle and Bamousa, Badr and Marrapodi, Maria Maddalena and Cicciu, Marco and Minervini, Giuseppe (2024) The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study. Technology and Health Care, 32 (4). pp. 2685-2696. ISSN 0928-7329, DOI https://doi.org/10.3233/THC-231814 <https://doi.org/10.3233/THC-231814>. https://doi.org/10.3233/THC-231814 10.3233/THC-231814
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RK Dentistry
spellingShingle RK Dentistry
Zeeshan, Tayyaba
Qamar, Zeeshan
Abdul, Nishath Sayed
Soman, Cristalle
Bamousa, Badr
Marrapodi, Maria Maddalena
Cicciu, Marco
Minervini, Giuseppe
The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
description BACKGROUND: Commercially available oral rinses contain active ingredients with concentration that is claimed by manufacturers to be effective as antiplaque agent. To date there has been no mention of the effect of oral rinse on the adherence of early plaque colonizers in plaque formation and the concentration to be used before/after meals. OBJECTIVE: The chief aim of the study was to evaluate microbial retention on the salivary pellicle on treatment with oral rinses (CHX & EO)/PS (mimicking after meals use of mouth wash/PS). METHODS: Noordini's Artifical Mouth model was used for developing the single species biofilm with early microbial colonizers of oral biofilm (A. viscosus, Strep. mitis and Strep. sanguinis respectively). The microbial retention on use of oral rinses comprising of CHX and EO as an active ingredients respectively was compared with Curcumin PS. For evaluating the microbial retention, the pellicle with microbial inoculation was developed on the glass beads in the mouth model. Subsequently the respective single specie biofilm was exposed to the mouth wash and PS after inoculation. It mimicked as use of mouth wash/PS after meals. The bacterial count in the dental biofilm was evaluated on serial dilution (CFU/ml). Sterile deionized water was used as a negative control. For qualitative analysis, Scanning electron microscope (SEM) was used to evaluate the microbial count. RESULTS: From the data it was observed that for the treatment of single species experimental biofilm with commercially available mouth rinses (CHX & EO) and PS (curcumin), there was significant retention for S.mitis, S.sanguinis and A.viscosus. There was no significant difference observed between PS and CHX treated single species biofilm. Whereas a significant difference was observed between EO treated biofilms and CHX/PS treated biofilms (p <= 0.05). CONCLUSION: It can be concluded from the results that curcumin PS and CHX should not be used after meals whereas EO containing mouth rinse can be used to maintain the oral mocroflora.
format Article
author Zeeshan, Tayyaba
Qamar, Zeeshan
Abdul, Nishath Sayed
Soman, Cristalle
Bamousa, Badr
Marrapodi, Maria Maddalena
Cicciu, Marco
Minervini, Giuseppe
author_facet Zeeshan, Tayyaba
Qamar, Zeeshan
Abdul, Nishath Sayed
Soman, Cristalle
Bamousa, Badr
Marrapodi, Maria Maddalena
Cicciu, Marco
Minervini, Giuseppe
author_sort Zeeshan, Tayyaba
title The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
title_short The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
title_full The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
title_fullStr The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
title_full_unstemmed The commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: An in vitro study
title_sort commercially available oral rinse vs. curcumin photosensitizers in an artificial mouth model mimicking their use after meals on early colonizers single species biofilm: an in vitro study
publisher IOS Press
publishDate 2024
url http://eprints.um.edu.my/47133/
https://doi.org/10.3233/THC-231814
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score 13.222552