Quantitative Applications of ATR-FTIR Spectroscopy with Chemometrics for the Estimation of Amikacin in Amikacin Sulphate Injections

• Main Authors: Chen, Chow Jie, Lokesh, Bontha Venkata Subrahmanya, Akowuah, Gabriel Akyirem
• Format: Article
• Published: Bentham Science Publishers 2024
• Subjects: RM Therapeutics. Pharmacology; RS Pharmacy and materia medica
• Online Access: http://eprints.um.edu.my/45846/; https://doi.org/10.2174/0115734110278516240129174949

Background Amikacin belongs to the class of aminoglycoside antibiotics used in the treatment of gram-negative bacterial infections. It is resistant to the aminoglycosides modifying enzymes, making it a clinically effective drug in multidrug-resistant infections.Methods In this study, a simple Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy was used for the quantification of amikacin in amikacin sulphate injection. The infrared spectra were generated in the spectral range of 4000-667 cm-1. The calibration curve was computed through TQ Analyst Pro edition software, and the partial least square regression analysis found the linearity in the range of 10-60% w/w.Results The best calibration results were obtained in the spectral region from 1040 to 1020 cm-1 with a correlation coefficient (r2) of 1.000. The residual mean standard error (RMSEC) value was 0.00235. The percent relative standard deviation (%RSD) values for intra-day and inter-day precision were less than 8.0. The percent relative error (%RE) values were calculated and found in between the range of 0.52 to 5.60. The percent recovery of the amikacin estimation was 113.09 +/- 4.27(n=3).Conclusion This validated method is considered a green method, which is suitable for the routine analysis of amikacin in amikacin sulphate injections.