Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study

• Main Authors: Oh, W.S., Suh, J.Y., Song, J.H., Ko, K.S., Jung, S.I., Peck, K.R., Lee, N.Y., Yang, Y.H., Chongthaleong, A., Chiu, C.H., Kamarulzaman, Adeeba, Parasakthi, N., Lalitha, M.K., Perera, J., Yee, T.T., Kumarasinghe, G., Carlos, C.C.
• Format: Article
• Published: Mary Ann Liebert 2004
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/4582/

Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC : 4 mug/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 —> Val in parE was the most common mutation. Data suggest that Lys137 —> Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 —> Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 —> Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 —> Val and Asn423 —> Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain(23)F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.