Erythrocarpines I–N, new limonoids from the barks of Chisocheton erythrocarpus and their neuroprotective effects against hydrogen peroxide in NG108–15 cells

• Main Authors: Ngadni, Muhammad Afiq, Chong, Soon-Lim, Kamarudin, Muhamad Noor Alfarizal, Hazni, Hazrina, Litaudon, Marc, Supratman, Unang, Awang, Khalijah
• Format: Article
• Published: Elsevier 2024
• Subjects: QD Chemistry; RM Therapeutics. Pharmacology; RS Pharmacy and materia medica
• Online Access: http://eprints.um.edu.my/44768/; https://doi.org/10.1016/j.fitote.2023.105765

A phytochemical study on the bark of Chisocheton erythrocarpus Hiern (Meliaceae) has led to the isolation of six new phragmalin-type limonoids named erythrocarpines I – N (1–6) along with one known limonoid, erythrocarpine F (7). Their structures were fully characterized by spectroscopic methods. The pre-treatment of NG108–15 cells with 1–5, 7 (2 h) demonstrated low to good protective effects against H2O2 exposure; 1 (83.77 ± 1.84 at 12.5 μM), 2 (69.07 ± 2.01 at 12.5 μM), 3 (80.38 ± 2.1 at 12.5 μM), 4 (62.33 ± 1.95 at 25 μM),5 (58.67 ± 1.85 at 50 μM) and 7 (66.07 ± 2.03 at 12.5 μM). Interestingly, 1 and 3 demonstrated comparable protective effects to positive control epigallocatechin gallate (EGCG) with similar cell viability capacity (≈ 80), having achieved that at lower concentration (12.5 μM) than EGCG (50 μM). Collectively, the results suggested the promising use of 1 and 3 as potential neuroprotective agents against hydrogen peroxide-induced cytotoxicity in neuronal model. © 2023 Elsevier B.V.