Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes
This study aimed to resolve if basement membrane (BM) collagen alpha chains undergo remodeling during oral. carcinogenesis. Using immunohistochemistry and transmission electron microscopy, we found that BMs in oral epithelial dysplasias (OED: mild, n=10; moderate, n=10; severe, n=10) and carcinoma i...
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my.um.eprints.43442013-01-10T00:17:02Z http://eprints.um.edu.my/4344/ Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes Tamamura, R. Nagatsuka, H. Siar, C.H. Katase, N. Naito, I. Sado, Y. Nagai, N. RK Dentistry This study aimed to resolve if basement membrane (BM) collagen alpha chains undergo remodeling during oral. carcinogenesis. Using immunohistochemistry and transmission electron microscopy, we found that BMs in oral epithelial dysplasias (OED: mild, n=10; moderate, n=10; severe, n=10) and carcinoma in situ (CIS) (n=10) differed from normal mucosa (n=6) and oral epithelial hyperplasia (n=5) in showing: (1) excessive lamina densa-like material ultrastructurally, and (2) stronger immunoexpression for alpha 5(IV) than for alpha 1(IV), alpha 2(IV), and alpha 6(IV) chains-findings that implicate these molecules' role as an adhesive template for the attachment and persistence of basal dysplastic cells. Incipient loss of BM integrity in CIS, where alpha 5(IV)/alpha 6(IV) chains were more frequently absent than alpha 1(IV)/alpha 2(IV) chains, suggests that alpha(IV) network disruption is crucial for progression of dysplastic cells into the extracellular compartment, marking transition into the invasive phase. In carcinomatous BM, the disappearance of alpha(IV) chains was more severe in poorly differentiated oral squamous cell carcinoma (OSCC) (n=10) than in well-differentiated OSCC (n=10). In all samples examined, alpha 3(IV) and alpha 4(IV) chains were absent. These findings taken together suggest that BM collagen-IV alpha chains undergo remodeling where selective increase and loss of these molecules are probably early and late events, respectively, during progression of oral dysplasia to cancer. 2006 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4344/1/Differential_expression_of_basement_membrane_collagen-IV_alpha_l_to_alpha_6_chains_during_oral_carcinogenes.pdf Tamamura, R. and Nagatsuka, H. and Siar, C.H. and Katase, N. and Naito, I. and Sado, Y. and Nagai, N. (2006) Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes. Virchows Archiv, 449 (3). pp. 358-366. ISSN 0945-6317 10.1007/s00428-006-0260-z |
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This study aimed to resolve if basement membrane (BM) collagen alpha chains undergo remodeling during oral. carcinogenesis. Using immunohistochemistry and transmission electron microscopy, we found that BMs in oral epithelial dysplasias (OED: mild, n=10; moderate, n=10; severe, n=10) and carcinoma in situ (CIS) (n=10) differed from normal mucosa (n=6) and oral epithelial hyperplasia (n=5) in showing: (1) excessive lamina densa-like material ultrastructurally, and (2) stronger immunoexpression for alpha 5(IV) than for alpha 1(IV), alpha 2(IV), and alpha 6(IV) chains-findings that implicate these molecules' role as an adhesive template for the attachment and persistence of basal dysplastic cells. Incipient loss of BM integrity in CIS, where alpha 5(IV)/alpha 6(IV) chains were more frequently absent than alpha 1(IV)/alpha 2(IV) chains, suggests that alpha(IV) network disruption is crucial for progression of dysplastic cells into the extracellular compartment, marking transition into the invasive phase. In carcinomatous BM, the disappearance of alpha(IV) chains was more severe in poorly differentiated oral squamous cell carcinoma (OSCC) (n=10) than in well-differentiated OSCC (n=10). In all samples examined, alpha 3(IV) and alpha 4(IV) chains were absent. These findings taken together suggest that BM collagen-IV alpha chains undergo remodeling where selective increase and loss of these molecules are probably early and late events, respectively, during progression of oral dysplasia to cancer. |
format |
Article |
author |
Tamamura, R. Nagatsuka, H. Siar, C.H. Katase, N. Naito, I. Sado, Y. Nagai, N. |
author_facet |
Tamamura, R. Nagatsuka, H. Siar, C.H. Katase, N. Naito, I. Sado, Y. Nagai, N. |
author_sort |
Tamamura, R. |
title |
Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes |
title_short |
Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes |
title_full |
Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes |
title_fullStr |
Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes |
title_full_unstemmed |
Differential expression of basement membrane collagen-IV alpha l to alpha 6 chains during oral carcinogenes |
title_sort |
differential expression of basement membrane collagen-iv alpha l to alpha 6 chains during oral carcinogenes |
publishDate |
2006 |
url |
http://eprints.um.edu.my/4344/1/Differential_expression_of_basement_membrane_collagen-IV_alpha_l_to_alpha_6_chains_during_oral_carcinogenes.pdf http://eprints.um.edu.my/4344/ |
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13.160551 |