Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract
This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scaven...
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2022
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my.um.eprints.433472023-11-10T02:13:24Z http://eprints.um.edu.my/43347/ Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract Rosmalena Putri, Nabilla Aretharify Yazid, Fatmawaty Ambarwati, Neneng Siti Silfi Omar, Hanita Ahmad, Islamudin R Medicine RS Pharmacy and materia medica This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl. An in vivo antioxidant with oxidative stress reduction was carried out for 10 days on 25 male Sprague-Dawley rats (divided into five groups). Every day, each group was given positive control, negative control, 5, 10, and 20 mg/200 gr of body weight (BW) of the extract. The blood plasma was taken for malondialdehyde analysis. A phytochemical identification of PEE revealed more compounds, such as flavonoids, alkaloids, steroids, essential oils, and tannin. PEE exhibits significant in vitro radical scavenging activity, with an IC 50 value of 126.695 μg/mL. In the in vivo antioxidant with oxidative stress reduction experiments, 5 mg/200 gr BW was the most effective dose, as evidenced by a considerable drop in malondialdehyde level (0.312 nmol/mL) after and before treatment. In conclusion, PPE has the potential to be developed as a herbal antioxidant based on in vitro and in vivo test results. © 2022 Journal of Advanced Pharmaceutical Technology & Research. Wolters Kluwer Medknow Publications 2022 Article PeerReviewed Rosmalena and Putri, Nabilla Aretharify and Yazid, Fatmawaty and Ambarwati, Neneng Siti Silfi and Omar, Hanita and Ahmad, Islamudin (2022) Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract. Journal of Advanced Pharmaceutical Technology and Research, 13 (2). 133 -137. ISSN 2231-4040, DOI https://doi.org/10.4103/japtr.japtr_16_22 <https://doi.org/10.4103/japtr.japtr_16_22>. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129448461&doi=10.4103%2fjaptr.japtr_16_22&partnerID=40&md5=60115e2f9008d692a46bf398c9edae8c 10.4103/japtr.japtr_16_22 |
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R Medicine RS Pharmacy and materia medica Rosmalena Putri, Nabilla Aretharify Yazid, Fatmawaty Ambarwati, Neneng Siti Silfi Omar, Hanita Ahmad, Islamudin Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
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This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl. An in vivo antioxidant with oxidative stress reduction was carried out for 10 days on 25 male Sprague-Dawley rats (divided into five groups). Every day, each group was given positive control, negative control, 5, 10, and 20 mg/200 gr of body weight (BW) of the extract. The blood plasma was taken for malondialdehyde analysis. A phytochemical identification of PEE revealed more compounds, such as flavonoids, alkaloids, steroids, essential oils, and tannin. PEE exhibits significant in vitro radical scavenging activity, with an IC 50 value of 126.695 μg/mL. In the in vivo antioxidant with oxidative stress reduction experiments, 5 mg/200 gr BW was the most effective dose, as evidenced by a considerable drop in malondialdehyde level (0.312 nmol/mL) after and before treatment. In conclusion, PPE has the potential to be developed as a herbal antioxidant based on in vitro and in vivo test results. © 2022 Journal of Advanced Pharmaceutical Technology & Research. |
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Rosmalena Putri, Nabilla Aretharify Yazid, Fatmawaty Ambarwati, Neneng Siti Silfi Omar, Hanita Ahmad, Islamudin |
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Rosmalena Putri, Nabilla Aretharify Yazid, Fatmawaty Ambarwati, Neneng Siti Silfi Omar, Hanita Ahmad, Islamudin |
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Rosmalena |
title |
Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
title_short |
Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
title_full |
Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
title_fullStr |
Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
title_full_unstemmed |
Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract |
title_sort |
phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (mentha piperita l.) leaves extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
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http://eprints.um.edu.my/43347/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129448461&doi=10.4103%2fjaptr.japtr_16_22&partnerID=40&md5=60115e2f9008d692a46bf398c9edae8c |
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