Specific, sensitive, and rapid diagnosis of active Toxoplasmosis by a Loop-Mediated Isothermal Amplification method using blood samples from patients

• Main Authors: Lau, Y.L., Meganathan, P., Sonaimuthu, P., Thiruvengadam, G., Nissapatorn, V., Chen, Y.
• Format: Article
• Language: English
• Published: 2010
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/4257/1/Lau-2010-Specific%2C_Sensitive.pdf; http://eprints.um.edu.my/4257/

Loop-mediated isothermal amplification (LAMP), a rapid nucleic acid amplification method, was developed for the clinical diagnosis of toxoplasmosis. Three LAMP assays based on the SAG1, SAG2, and B1 genes of Toxoplasma gondii were developed. The sensitivities and specificities of the LAMP assays wer...