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In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis

Clinical efficacy of chemotherapy is often compromised by diabetogenic glucose on colorectal cancer (CRC). High glucose has been shown to diminish the cytotoxicity of anticancer drugs. The issue can potentially be addressed with natural products. Recently, we revealed that Ganoderma neo-japonicum ex...

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Main Authors: Lau, Meng-Fei, Chua, Kek Heng, Sabaratnam, Vikineswary, Kuppusamy, Umah Rani
Format: Article
Published: Taylor & Francis 2022
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RC Internal medicine
Online Access:http://eprints.um.edu.my/42454/
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spelling my.um.eprints.424542023-10-09T06:34:12Z http://eprints.um.edu.my/42454/ In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis Lau, Meng-Fei Chua, Kek Heng Sabaratnam, Vikineswary Kuppusamy, Umah Rani RC Internal medicine Clinical efficacy of chemotherapy is often compromised by diabetogenic glucose on colorectal cancer (CRC). High glucose has been shown to diminish the cytotoxicity of anticancer drugs. The issue can potentially be addressed with natural products. Recently, we revealed that Ganoderma neo-japonicum exhibits inhibitory activities against human colonic carcinoma cells. In this study, the impacts of hexane fraction (Hex, sterol-enriched) and chloroform fraction (Chl, terpenoid-enriched) were further elucidated. The cellular responses, including oxidative stress, cell cycle, and apoptosis were compared between the presence of normal glucose (NG, 5.5 mM) and high glucose (HG, 25 mM). HG promoted cell viability with concomitant elevation of GSH level. Both Hex and Chl fractions stimulated NO production, in addition, induced cell cycle arrest. The apoptotic effect of Hex fraction was glucose-dependent, but Chl fraction triggered apoptosis with an equivalent extent in NG and HG conditions. Overall, the active fractions from G. neo-japonicum show therapeutic potential in managing hyperglycemia-associated CRC. Taylor & Francis 2022-03 Article PeerReviewed Lau, Meng-Fei and Chua, Kek Heng and Sabaratnam, Vikineswary and Kuppusamy, Umah Rani (2022) In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis. Nutrition and Cancer-An International Journal, 74 (3). pp. 978-995. ISSN 0163-5581, DOI https://doi.org/10.1080/01635581.2021.1931701 <https://doi.org/10.1080/01635581.2021.1931701>. 10.1080/01635581.2021.1931701
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RC Internal medicine
spellingShingle RC Internal medicine
Lau, Meng-Fei
Chua, Kek Heng
Sabaratnam, Vikineswary
Kuppusamy, Umah Rani
In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
description Clinical efficacy of chemotherapy is often compromised by diabetogenic glucose on colorectal cancer (CRC). High glucose has been shown to diminish the cytotoxicity of anticancer drugs. The issue can potentially be addressed with natural products. Recently, we revealed that Ganoderma neo-japonicum exhibits inhibitory activities against human colonic carcinoma cells. In this study, the impacts of hexane fraction (Hex, sterol-enriched) and chloroform fraction (Chl, terpenoid-enriched) were further elucidated. The cellular responses, including oxidative stress, cell cycle, and apoptosis were compared between the presence of normal glucose (NG, 5.5 mM) and high glucose (HG, 25 mM). HG promoted cell viability with concomitant elevation of GSH level. Both Hex and Chl fractions stimulated NO production, in addition, induced cell cycle arrest. The apoptotic effect of Hex fraction was glucose-dependent, but Chl fraction triggered apoptosis with an equivalent extent in NG and HG conditions. Overall, the active fractions from G. neo-japonicum show therapeutic potential in managing hyperglycemia-associated CRC.
format Article
author Lau, Meng-Fei
Chua, Kek Heng
Sabaratnam, Vikineswary
Kuppusamy, Umah Rani
author_facet Lau, Meng-Fei
Chua, Kek Heng
Sabaratnam, Vikineswary
Kuppusamy, Umah Rani
author_sort Lau, Meng-Fei
title In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
title_short In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
title_full In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
title_fullStr In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
title_full_unstemmed In vitro anti-colorectal cancer potential of the medicinal mushroom Ganoderma neo-japonicum Imazeki in hyperglycemic condition: Impact on oxidative stress, cell cycle and apoptosis
title_sort in vitro anti-colorectal cancer potential of the medicinal mushroom ganoderma neo-japonicum imazeki in hyperglycemic condition: impact on oxidative stress, cell cycle and apoptosis
publisher Taylor & Francis
publishDate 2022
url http://eprints.um.edu.my/42454/
_version_ 1781704645845975040
score 13.159267

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