Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015

Enterovirus D68 (EV-D68) is an emerging respiratory pathogen since the 2014 outbreak in the United States. A low level of virus circulation has been reported in Kuala Lumpur, Malaysia, in the past. However, the extent of the infection in Malaysia is not known. In the present study, we determine the...

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Main Authors: Chan, Yoke Fun, Sam, Jamal I-Ching, Nayan, Elena, Tan, Xiu Hui, Yogarajah, Thinesshwary
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Published: Wiley 2022
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Online Access:http://eprints.um.edu.my/42423/
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spelling my.um.eprints.424232023-10-09T12:59:32Z http://eprints.um.edu.my/42423/ Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015 Chan, Yoke Fun Sam, Jamal I-Ching Nayan, Elena Tan, Xiu Hui Yogarajah, Thinesshwary R Medicine Enterovirus D68 (EV-D68) is an emerging respiratory pathogen since the 2014 outbreak in the United States. A low level of virus circulation has been reported in Kuala Lumpur, Malaysia, in the past. However, the extent of the infection in Malaysia is not known. In the present study, we determine the seroepidemiology of EV-D68 in Kuala Lumpur, Malaysia, before and after the United States outbreak in August 2014. A luciferase-based seroneutralization test was developed using a clone-derived prototype Fermon strain carrying a nanoluciferase marker. We screened the neutralization capacity of 450 serum samples from children and adults (1-89 years old) collected between 2013 and 2015. EV-D68 seropositivity increased with age, with children aged 1-3 showing significantly lower seroprevalence compared to adults. Multivariate analysis showed that older age groups 13-49 years (odds ratio OR] = 4.78; 95% confidence interval CI] = 2.69-8.49; p < 0.0001) and >= 50 years (OR = 3.83; 95% CI = 2.19-6.68; p < 0.0001) were more likely to be EV-D68 seropositive than children <13 years. Sampling post-September 2014 compared to pre-Sept 2014 also predicted seropositivity (OR = 1.66; 95% CI = 1.04-2.65). The presence of neutralizing antibodies against EV-D68 in the study population suggests that EV-D68 was circulating before 2014. A higher seropositivity post-September 2014 suggests that Malaysia also experienced an upsurge in EV-D68 infections after the United States outbreaks in August 2014. A low seropositivity rate observed in children, especially those aged 1-3 years old, suggests that they are at risk and should be prioritized for future vaccination. Wiley 2022-06 Article PeerReviewed Chan, Yoke Fun and Sam, Jamal I-Ching and Nayan, Elena and Tan, Xiu Hui and Yogarajah, Thinesshwary (2022) Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015. Journal Of Medical Virology, 94 (6). pp. 2607-2612. ISSN 0146-6615, DOI https://doi.org/10.1002/jmv.27381 <https://doi.org/10.1002/jmv.27381>. 10.1002/jmv.27381
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Chan, Yoke Fun
Sam, Jamal I-Ching
Nayan, Elena
Tan, Xiu Hui
Yogarajah, Thinesshwary
Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
description Enterovirus D68 (EV-D68) is an emerging respiratory pathogen since the 2014 outbreak in the United States. A low level of virus circulation has been reported in Kuala Lumpur, Malaysia, in the past. However, the extent of the infection in Malaysia is not known. In the present study, we determine the seroepidemiology of EV-D68 in Kuala Lumpur, Malaysia, before and after the United States outbreak in August 2014. A luciferase-based seroneutralization test was developed using a clone-derived prototype Fermon strain carrying a nanoluciferase marker. We screened the neutralization capacity of 450 serum samples from children and adults (1-89 years old) collected between 2013 and 2015. EV-D68 seropositivity increased with age, with children aged 1-3 showing significantly lower seroprevalence compared to adults. Multivariate analysis showed that older age groups 13-49 years (odds ratio OR] = 4.78; 95% confidence interval CI] = 2.69-8.49; p < 0.0001) and >= 50 years (OR = 3.83; 95% CI = 2.19-6.68; p < 0.0001) were more likely to be EV-D68 seropositive than children <13 years. Sampling post-September 2014 compared to pre-Sept 2014 also predicted seropositivity (OR = 1.66; 95% CI = 1.04-2.65). The presence of neutralizing antibodies against EV-D68 in the study population suggests that EV-D68 was circulating before 2014. A higher seropositivity post-September 2014 suggests that Malaysia also experienced an upsurge in EV-D68 infections after the United States outbreaks in August 2014. A low seropositivity rate observed in children, especially those aged 1-3 years old, suggests that they are at risk and should be prioritized for future vaccination.
format Article
author Chan, Yoke Fun
Sam, Jamal I-Ching
Nayan, Elena
Tan, Xiu Hui
Yogarajah, Thinesshwary
author_facet Chan, Yoke Fun
Sam, Jamal I-Ching
Nayan, Elena
Tan, Xiu Hui
Yogarajah, Thinesshwary
author_sort Chan, Yoke Fun
title Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
title_short Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
title_full Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
title_fullStr Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
title_full_unstemmed Seroepidemiology of enterovirus D68 infection in Kuala Lumpur, Malaysia between 2013 and 2015
title_sort seroepidemiology of enterovirus d68 infection in kuala lumpur, malaysia between 2013 and 2015
publisher Wiley
publishDate 2022
url http://eprints.um.edu.my/42423/
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score 13.222552