The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability
Purpose/ Aim The main purpose of this work is to study the cellular viability effect of irradiated riboflavin in cultured human tenon fibroblasts. Materials and Methods The tenon tissue was harvested from a patient undergoing strabismus surgery. The human tenon fibroblast cell culture and isolation...
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my.um.eprints.423872023-10-10T04:47:05Z http://eprints.um.edu.my/42387/ The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability See, Wendy Yen Nee Ismail, Fazliana Kadir, Siti Hamimah Sheikh Abdul Subrayan, Visvaraja RE Ophthalmology Purpose/ Aim The main purpose of this work is to study the cellular viability effect of irradiated riboflavin in cultured human tenon fibroblasts. Materials and Methods The tenon tissue was harvested from a patient undergoing strabismus surgery. The human tenon fibroblast cell culture and isolation were performed according to the standard laboratory cell culturing protocol. The cells were divided into three groups: control, treatment with irradiated and non-irradiated riboflavin. There were five different concentrations (0.00156%, 0.003125%, 0.00625%, 0.0125%, 0.025%) in each group of riboflavin. The fibroblasts were treated with riboflavin and the cellular viability was assessed at 24-hour and 48-hour post treatment with MTT 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide colorimetric assay. The absorbance values were analysed using Magellan microplate reader data analysis. A triplicate of readings was taken. The data were presented as mean +/- standard deviation of the triplicates. Statistical analysis was performed with Statistical Package for Social Sciences (SPSS) analysis version 23. Results Irradiated riboflavin caused a concentration-dependent cell death in human tenon fibroblast cell culture (p < .05). The antiproliferative difference between irradiated and non-irradiated riboflavin was significant up to 48 hours (p < .05). Post hoc multiple comparisons showed higher concentrations of irradiated riboflavin (0.0125% and 0.025%) caused more reduction in cellular viability in human tenon fibroblast cells (p < .05). The duration of treatment is not a causative factor in this study. Conclusions This pilot experiment demonstrated that irradiated riboflavin induced cell death in human tenon fibroblast culture in a concentration-dependent manner, but is not time-dependent. Further exploratory investigations should be performed to determine the mechanism of cell death. We postulate that apoptosis occurred in these irradiated riboflavin-treated cells. TAYLOR & FRANCIS INC 2022-04-03 Article PeerReviewed See, Wendy Yen Nee and Ismail, Fazliana and Kadir, Siti Hamimah Sheikh Abdul and Subrayan, Visvaraja (2022) The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability. Current Eye Research, 47 (4). pp. 525-530. ISSN 0271-3683, DOI https://doi.org/10.1080/02713683.2021.2011326 <https://doi.org/10.1080/02713683.2021.2011326>. 10.1080/02713683.2021.2011326 |
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RE Ophthalmology See, Wendy Yen Nee Ismail, Fazliana Kadir, Siti Hamimah Sheikh Abdul Subrayan, Visvaraja The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
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Purpose/ Aim The main purpose of this work is to study the cellular viability effect of irradiated riboflavin in cultured human tenon fibroblasts. Materials and Methods The tenon tissue was harvested from a patient undergoing strabismus surgery. The human tenon fibroblast cell culture and isolation were performed according to the standard laboratory cell culturing protocol. The cells were divided into three groups: control, treatment with irradiated and non-irradiated riboflavin. There were five different concentrations (0.00156%, 0.003125%, 0.00625%, 0.0125%, 0.025%) in each group of riboflavin. The fibroblasts were treated with riboflavin and the cellular viability was assessed at 24-hour and 48-hour post treatment with MTT 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide colorimetric assay. The absorbance values were analysed using Magellan microplate reader data analysis. A triplicate of readings was taken. The data were presented as mean +/- standard deviation of the triplicates. Statistical analysis was performed with Statistical Package for Social Sciences (SPSS) analysis version 23. Results Irradiated riboflavin caused a concentration-dependent cell death in human tenon fibroblast cell culture (p < .05). The antiproliferative difference between irradiated and non-irradiated riboflavin was significant up to 48 hours (p < .05). Post hoc multiple comparisons showed higher concentrations of irradiated riboflavin (0.0125% and 0.025%) caused more reduction in cellular viability in human tenon fibroblast cells (p < .05). The duration of treatment is not a causative factor in this study. Conclusions This pilot experiment demonstrated that irradiated riboflavin induced cell death in human tenon fibroblast culture in a concentration-dependent manner, but is not time-dependent. Further exploratory investigations should be performed to determine the mechanism of cell death. We postulate that apoptosis occurred in these irradiated riboflavin-treated cells. |
format |
Article |
author |
See, Wendy Yen Nee Ismail, Fazliana Kadir, Siti Hamimah Sheikh Abdul Subrayan, Visvaraja |
author_facet |
See, Wendy Yen Nee Ismail, Fazliana Kadir, Siti Hamimah Sheikh Abdul Subrayan, Visvaraja |
author_sort |
See, Wendy Yen Nee |
title |
The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
title_short |
The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
title_full |
The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
title_fullStr |
The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
title_full_unstemmed |
The effect of irradiated riboflavin in human tenon's fibroblast - A study on cellular viability |
title_sort |
effect of irradiated riboflavin in human tenon's fibroblast - a study on cellular viability |
publisher |
TAYLOR & FRANCIS INC |
publishDate |
2022 |
url |
http://eprints.um.edu.my/42387/ |
_version_ |
1781704636602777600 |
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13.18916 |