Development of a rapid DNA extraction method and one-step nested PCR for the detection of Naegleria fowleri from the environment

• Main Authors: Ahmad, A.F., Lonnen, J., Andrew, P.W., Kilvington, S.
• Format: Article
• Language: English
• Published: 2011
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/4190/1/Ahmad-2011-Development_of_a_rap.pdf; http://eprints.um.edu.my/4190/

Naegleria fowleri is a small free-living amoebo-flagellate found in natural and manmade thermal aquatic habitats worldwide. The organism is pathogenic to man causing fatal primary amoebic meningoencephalitis (PAM). Infection typically results from bathing in contaminated water and is usually fatal....