Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum
There is a pressing demand for new sustainable eco-friendly approaches to producing green energy worldwide. This study represents the novel production of biodiesel feedstock from the medicinal mushroom Ganoderma lucidum QRS 5120 using state-of-the-art biotechnology tools. Response surface methodolog...
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my.um.eprints.413172023-09-19T01:29:30Z http://eprints.um.edu.my/41317/ Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum Jahis, Besek Mariam Mohamad Ilham, Zul Supramani, Sugenendran Sohedein, Mohamad Nor Azzimi Ibrahim, Mohamad Faizal Abd-Aziz, Suraini Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad GE Environmental Sciences There is a pressing demand for new sustainable eco-friendly approaches to producing green energy worldwide. This study represents the novel production of biodiesel feedstock from the medicinal mushroom Ganoderma lucidum QRS 5120 using state-of-the-art biotechnology tools. Response surface methodology (RSM) was used to enhance G. lucidum production in a repeated-batch fermentation strategy. By referring to the broth replacement ratio (BRR) and broth replacement time point (BRTP), RSM that was formulated using a central composite design (CCD) resulted in a significant model for all tested variables, which are exopolysaccharide (EPS), endopolysaccharide (ENS) and biomass, with BRR (%) of 60, 75 and 90, and BRTP (days) of 11, 13 and 15. The model was validated using the optimised conditions, and the results showed 4.21 g/L of EPS (BRR 77.46% and BRTP 12 days), 2.44 g/L of ENS (BRR 60% and BRTP 12.85 days), and 34.32 g/L of biomass (BRR 89.52% and BRTP 10.96 days) were produced. Biomass produced from the G. lucidum was subsequently used as feedstock for biodiesel production. Approximately 20.36% of lipid was successfully extracted from the dried G. lucidum biomass via a solvent extraction and subsequently converted to Ganodiesel through a transesterification process. The Ganodiesel produced fulfilled most of the international standards, i.e., US (ASTM D6751-08) and EU (EN 14214). Overall, this study demonstrates the optimised G. lucidum production and its lipid production as a new biodiesel feedstock. MDPI 2022-09 Article PeerReviewed Jahis, Besek Mariam Mohamad and Ilham, Zul and Supramani, Sugenendran and Sohedein, Mohamad Nor Azzimi and Ibrahim, Mohamad Faizal and Abd-Aziz, Suraini and Rowan, Neil and Wan-Mohtar, Wan Abd Al Qadr Imad (2022) Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum. Sustainability, 14 (17). ISSN 2071-1050, DOI https://doi.org/10.3390/su141710764 <https://doi.org/10.3390/su141710764>. 10.3390/su141710764 |
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GE Environmental Sciences Jahis, Besek Mariam Mohamad Ilham, Zul Supramani, Sugenendran Sohedein, Mohamad Nor Azzimi Ibrahim, Mohamad Faizal Abd-Aziz, Suraini Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
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There is a pressing demand for new sustainable eco-friendly approaches to producing green energy worldwide. This study represents the novel production of biodiesel feedstock from the medicinal mushroom Ganoderma lucidum QRS 5120 using state-of-the-art biotechnology tools. Response surface methodology (RSM) was used to enhance G. lucidum production in a repeated-batch fermentation strategy. By referring to the broth replacement ratio (BRR) and broth replacement time point (BRTP), RSM that was formulated using a central composite design (CCD) resulted in a significant model for all tested variables, which are exopolysaccharide (EPS), endopolysaccharide (ENS) and biomass, with BRR (%) of 60, 75 and 90, and BRTP (days) of 11, 13 and 15. The model was validated using the optimised conditions, and the results showed 4.21 g/L of EPS (BRR 77.46% and BRTP 12 days), 2.44 g/L of ENS (BRR 60% and BRTP 12.85 days), and 34.32 g/L of biomass (BRR 89.52% and BRTP 10.96 days) were produced. Biomass produced from the G. lucidum was subsequently used as feedstock for biodiesel production. Approximately 20.36% of lipid was successfully extracted from the dried G. lucidum biomass via a solvent extraction and subsequently converted to Ganodiesel through a transesterification process. The Ganodiesel produced fulfilled most of the international standards, i.e., US (ASTM D6751-08) and EU (EN 14214). Overall, this study demonstrates the optimised G. lucidum production and its lipid production as a new biodiesel feedstock. |
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Article |
author |
Jahis, Besek Mariam Mohamad Ilham, Zul Supramani, Sugenendran Sohedein, Mohamad Nor Azzimi Ibrahim, Mohamad Faizal Abd-Aziz, Suraini Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad |
author_facet |
Jahis, Besek Mariam Mohamad Ilham, Zul Supramani, Sugenendran Sohedein, Mohamad Nor Azzimi Ibrahim, Mohamad Faizal Abd-Aziz, Suraini Rowan, Neil Wan-Mohtar, Wan Abd Al Qadr Imad |
author_sort |
Jahis, Besek Mariam Mohamad |
title |
Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
title_short |
Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
title_full |
Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
title_fullStr |
Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
title_full_unstemmed |
Ganodiesel: A new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
title_sort |
ganodiesel: a new biodiesel feedstock from biomass of the mushroom ganoderma lucidum |
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MDPI |
publishDate |
2022 |
url |
http://eprints.um.edu.my/41317/ |
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1778161655347150848 |
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13.160551 |