Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia

Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were...

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Bibliographic Details
Main Authors: Lim, Y.A.L., Ramasame, S.D., Mahdy, M.A.K., Sulaiman, W.Y.W., Smith, H.V.
Format: Article
Language:English
Published: 2009
Subjects:
Online Access:http://eprints.um.edu.my/4110/1/Lim-2009-Detection_and_molecu.pdf
http://eprints.um.edu.my/4110/
http://www.ncbi.nlm.nih.gov/pubmed/19705155
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Summary:Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water.