Detection and molecular characterization of Giardia isolated from recreational lake water in Malaysia
Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were...
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Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
2009
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Subjects: | |
Online Access: | http://eprints.um.edu.my/4110/1/Lim-2009-Detection_and_molecu.pdf http://eprints.um.edu.my/4110/ http://www.ncbi.nlm.nih.gov/pubmed/19705155 |
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Summary: | Nine 50-l surface water samples from a Malaysian recreational lake were examined microscopically using an immunomagnetisable separation-immunofluorescent method. No Cryptosporidium oocysts were detected, but 77.8 of samples contained low numbers of Giardia cysts (range, 0.17-1.1 cysts/l), which were genetically characterised by SSU rRNA gene sequencing. Genotype analyses indicated the presence of Giardia duodenalis assemblage A suggesting potential risk to public health. The present study represents the first contribution to our knowledge of G. duodenalis assemblages in Malaysian recreational water. |
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