Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli

• Main Authors: Fong, M.Y., Lau, Y.L., Init, I., Jamaiah, I., Anuar, A.K., Rahmah, N.
• Format: Article
• Language: English
• Published: 2003
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/4109/1/Recombinant_expression_of_Toxocara_canis_excretory-secretory_antigen_TES-120_in_Escherichia_coli..pdf; http://eprints.um.edu.my/4109/; http://www.ncbi.nlm.nih.gov/pubmed/15115078

The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.