Cloning and expression of Toxoplasma gondii dense granular protein 4 (GRA4) in Pichia pastoris

• Main Authors: Lau, Y.L., Hasan, M.T., Thiruvengadam, G., Idris, M.M., Init, I.
• Format: Article
• Language: English
• Published: 2010
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/4094/1/Cloning_and_expression_of_Toxoplasma_gondii_dense_granular_protein_4_%28GRA4%29_in_Pichia_pastoris.pdf; http://eprints.um.edu.my/4094/; http://www.ncbi.nlm.nih.gov/pubmed/21399595

GRA4 of Toxoplasma gondii has been shown to prompt IgG, IgM and IgA responses in previous studies and is thus considered one of the major immunogenic proteins from T. gondii that can be used for both diagnostics purposes and vaccine development. This study seeks to clone and express the GRA4 in Pich...