Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei
Trypanosoma brucei parasites are flagellated kinetoplastid protozoan which is responsible for Human African Trypanosomiasis (HAT). Current chemotherapy drugs have a number of side effects and drug resistance has emerged as a major issue in current treatment. Active bisindole alkaloid compound ochrol...
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my.um.eprints.409062023-09-25T04:42:05Z http://eprints.um.edu.my/40906/ Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei Norhayati, Ibrahim Nurhayati, Z. A. Getha, K. Haffiz, J. Muhd Adiratna, M. R. GE Environmental Sciences QK Botany R Medicine Trypanosoma brucei parasites are flagellated kinetoplastid protozoan which is responsible for Human African Trypanosomiasis (HAT). Current chemotherapy drugs have a number of side effects and drug resistance has emerged as a major issue in current treatment. Active bisindole alkaloid compound ochrolifuanine was previously isolated from the leaves of Dyera costulata. In vitro antitrypanosomal activity of ochrolifuanine against Trypanosoma brucei brucei strain BS221 showed strong activity with an IC50 value of 0.05 +/- 0.01 mu g/ml. We compared the effect of ochrolifuanine and reference compound staurosporine in T. b. brucei apoptosis. The apoptosis-inducing activity of ochrolifuanine was evaluated using TUNEL assay and cell cycle analysis. Trypanosoma brucei brucei was shown to undergo apoptotic cells death as demonstrated by the appearance of several conical hallmarks of apoptosis. Ochrolifuanine was found to induce apoptosis in parasites in a dose-and time-dependent manner. The cell cycle study revealed 0.025 and 0.05 mu g/ml of ochrolifuanine arrested the growth of T. b. brucei at two different growth phases (G0/G1 and in S phases). While at concentration 0.10 mu g/ml arrested at the G2/M phase. In conclusion, the results indicate that ochrolifuanine displayed an antitrypanosomal effect on T. b. brucei by inducing apoptosis cell death and causing the arrest of parasite cells at different growth phases. The results suggested that ochrolifuanine may be a promising lead compound for the development of new chemotherapies for African trypanosomiasis. Malaysian Society of Parasitology and Tropical Medicine 2022-09 Article PeerReviewed Norhayati, Ibrahim and Nurhayati, Z. A. and Getha, K. and Haffiz, J. Muhd and Adiratna, M. R. (2022) Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei. Tropical Biomedicine, 39 (3). pp. 321-327. ISSN 2521-9855, DOI https://doi.org/10.47665/tb.39.3.003 <https://doi.org/10.47665/tb.39.3.003>. 10.47665/tb.39.3.003 |
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GE Environmental Sciences QK Botany R Medicine Norhayati, Ibrahim Nurhayati, Z. A. Getha, K. Haffiz, J. Muhd Adiratna, M. R. Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
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Trypanosoma brucei parasites are flagellated kinetoplastid protozoan which is responsible for Human African Trypanosomiasis (HAT). Current chemotherapy drugs have a number of side effects and drug resistance has emerged as a major issue in current treatment. Active bisindole alkaloid compound ochrolifuanine was previously isolated from the leaves of Dyera costulata. In vitro antitrypanosomal activity of ochrolifuanine against Trypanosoma brucei brucei strain BS221 showed strong activity with an IC50 value of 0.05 +/- 0.01 mu g/ml. We compared the effect of ochrolifuanine and reference compound staurosporine in T. b. brucei apoptosis. The apoptosis-inducing activity of ochrolifuanine was evaluated using TUNEL assay and cell cycle analysis. Trypanosoma brucei brucei was shown to undergo apoptotic cells death as demonstrated by the appearance of several conical hallmarks of apoptosis. Ochrolifuanine was found to induce apoptosis in parasites in a dose-and time-dependent manner. The cell cycle study revealed 0.025 and 0.05 mu g/ml of ochrolifuanine arrested the growth of T. b. brucei at two different growth phases (G0/G1 and in S phases). While at concentration 0.10 mu g/ml arrested at the G2/M phase. In conclusion, the results indicate that ochrolifuanine displayed an antitrypanosomal effect on T. b. brucei by inducing apoptosis cell death and causing the arrest of parasite cells at different growth phases. The results suggested that ochrolifuanine may be a promising lead compound for the development of new chemotherapies for African trypanosomiasis. |
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Article |
author |
Norhayati, Ibrahim Nurhayati, Z. A. Getha, K. Haffiz, J. Muhd Adiratna, M. R. |
author_facet |
Norhayati, Ibrahim Nurhayati, Z. A. Getha, K. Haffiz, J. Muhd Adiratna, M. R. |
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Norhayati, Ibrahim |
title |
Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
title_short |
Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
title_full |
Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
title_fullStr |
Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
title_full_unstemmed |
Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei |
title_sort |
evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from dyera costulata (miq.) hook.f against trypanosoma brucei brucei |
publisher |
Malaysian Society of Parasitology and Tropical Medicine |
publishDate |
2022 |
url |
http://eprints.um.edu.my/40906/ |
_version_ |
1778161625776259072 |
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13.18916 |