Kinetic studies of immobilized wheat-based alpha-naphthyl acetate esterase in the detection of organophosphate pesticides

• Main Authors: Nageib, Adilah Mohamed, Jameel, Ahmad Tariq, Ahmad, Farah
• Format: Article
• Published: Wiley 2022
• Subjects: TP Chemical technology
• Online Access: http://eprints.um.edu.my/40810/

Organophosphate pesticides could inhibit the activity of alpha-naphthyl acetate esterase (ANAE), which could be the fundamental for biosensor applications. k-carrageenan was used for immobilization of ANAE extracted from wheat-based sources. The activity of ANAE was assessed for optimum activity at a range of temperature and pH. Enzyme kinetic studies were performed using Michaelis-Menten kinetic parameters from Lineweaver-Burk, Langmuir, and Eddie-Hofstee. The optimization studies showed that the relative activity of the enzyme remained high, even above 50 degrees C. k(d) obtained was very low, which indicates the high sensitivity of ANAE towards pesticides. The kinetic reaction of ANAE showed the promising application of biosensors in the detection of pesticide.