Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients
Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time poly...
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my.um.eprints.40382012-12-05T03:39:03Z http://eprints.um.edu.my/4038/ Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients Othman, N. Mohamed, Z. Verweij, J.J. Huat, L.B. Olivos-Garcia, A. Yeng, C. Noordin, R. RK Dentistry Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7) pus samples, when compared with 14 of 30 (46.7) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/4038/1/Application_of_Real-Time_Polymerase_Chain_Reaction_in_Detection_of_Entamoeba_histolytica_in_Pus_Aspirates_of_Liver_Abscess_Patients.pdf Othman, N. and Mohamed, Z. and Verweij, J.J. and Huat, L.B. and Olivos-Garcia, A. and Yeng, C. and Noordin, R. (2010) Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients. Foodborne Pathogens and Disease, 7 (6). pp. 637-641. ISSN 1535-3141 10.1089/fpd.2009.0427 |
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RK Dentistry Othman, N. Mohamed, Z. Verweij, J.J. Huat, L.B. Olivos-Garcia, A. Yeng, C. Noordin, R. Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
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Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7) pus samples, when compared with 14 of 30 (46.7) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess. |
format |
Article |
author |
Othman, N. Mohamed, Z. Verweij, J.J. Huat, L.B. Olivos-Garcia, A. Yeng, C. Noordin, R. |
author_facet |
Othman, N. Mohamed, Z. Verweij, J.J. Huat, L.B. Olivos-Garcia, A. Yeng, C. Noordin, R. |
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Othman, N. |
title |
Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
title_short |
Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
title_full |
Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
title_fullStr |
Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
title_full_unstemmed |
Application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
title_sort |
application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients |
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2010 |
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http://eprints.um.edu.my/4038/1/Application_of_Real-Time_Polymerase_Chain_Reaction_in_Detection_of_Entamoeba_histolytica_in_Pus_Aspirates_of_Liver_Abscess_Patients.pdf http://eprints.um.edu.my/4038/ |
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1643687247015313408 |
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13.160551 |