PCR fingerprinting of Blastocystis isolated from symptomatic and asymptomatic human hosts

• Main Authors: Tan, Tian Chye, Suresh, K.G., Thong, Kwai Lin, Smith, H.V.
• Format: Article
• Language: English
• Published: Springer Verlag (Germany) 2006
• Subjects: Q Science (General); QH Natural history; R Medicine
• Online Access: http://eprints.um.edu.my/4022/1/Tan-2006-PCR_fingerprinting_o.pdf; http://eprints.um.edu.my/4022/; https://doi.org/10.1007/s00436-006-0177-0

Genomic DNA from 16 Blastocystis hominis isolates comprising of eight asymptomatic isolates (A1-A8) and eight symptomatic isolates (S1-S8) was amplified by arbitrarily primed polymerase chain reaction (AP-PCR) using 38 arbitrary 10-mer primers. Six primers (A10, B5, C20, D1, F6, and F10) generated r...