Simultaneous differential detection of human pathogenic and nonpathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genes

• Main Authors: Teh, C.S.J., Chua, K.H., Thong, Kwai Lin
• Format: Article
• Published: Blackwell Publishing 2010
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3849/

Aims: To develop a multiplex PCR targeting the gyrB and pntA genes for Vibrio species differentiation. Methods and Results: Four pairs of primers targeting gyrB gene of Vibrios at genus level and pntA gene of Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus were designed. This PCR method...